新生期大鼠反复惊厥上调大脑皮质可塑性相关基因-1蛋白水平  

Upregulated expression of plasticity-related gene 1 protein in cerebral cortex in a rat model of recurrent neonatal seizures

作　　者：肖倬君[1] 倪宏[1] 王浙东[1] 

机构地区：[1]苏州大学附属儿童医院儿科医学研究所,苏州215003

出　　处：《中华行为医学与脑科学杂志》2010年第6期504-506,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30870808）;江苏省高校自然科学基金项目（07KJB320103）

摘　　要：目的 探索可塑性相关基因-1（plasticity related gene 1,PRG-1）在反复惊厥大鼠大脑皮质中的动态表达及其意义.方法 用三氟乙醚使新生第6天的SD大鼠呈现反复惊厥状态,每天持续30min,连续6d,分别于末次惊厥后3h,12h,48 h和14d取大脑皮质,并每个时间点设未做任何干预对照组,用免疫印迹方法观察PRG-1蛋白表达.结果 末次惊厥后3 h,12h,48 h、14d大脑皮质可塑性相关基因-1的表达,对照组分别为1.363±0.742、1.278±0.687、0.763±0.374、1.004±0.113,实验组分为1.818±1.093、1.562±0.782、1.024±0.510、1.378±0.279.3 h、12h、48 h大脑皮质PRG-1的表达在实验组和对照组无统计学意义（t=0.843,0.668,1.011,均P＞0.05）.14d大脑皮质PRG-1的表达,实验组明显高于对照组（t=3.041,P＜0.05）.结论 PRG-1在新生大鼠反复长程惊厥后的远期表达增多,提示参与发育期惊厥性脑损伤的病理生理机制.Objective To explore the dynamic expression of a new phospholipid phosphatase, plasticity related gene 1 （ PRG-1 ） in cerebral cortex following recurrent neonatal seizures. Methods A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 （ P6 ）. Rats were assigned into the recurrent-seizure group （seizures induced in six consecutive days） and the control group. At 3 h,12 h,48 h, 14d after the last convulsion,PRG-1 protein level in cerebral cortex was detected by western blot method. Results At 3 h,12 h,48 h,14d after the last convulsion in control group,the expression of PRG-1 in cerebral cortex respectively was 1. 363 ± 0. 742,1. 278 ± 0. 687,0. 763 ± 0. 374,1. 004 ± 0. 113, in experimental group , the expression of PRG-1 in cerebral cortex respectively was 1.818 ± 1. 093,1. 562 ± 0. 782,1. 024 ± 0.510,1. 378 ± 0. 279. At 3 h, 12 h,48 h,the expression of PRG-1 in cerebral cortex was not significantly different between the experimental and control group （ t = 0. 843,0. 668,1.011 ,all P 〉 0.05 ）. However, at 14 d, the level of PRG-1 protein in cerebral cortex of experimental group was significantly higher than that of control （ t = 3. 041, P 〈 0.05 ）. Conclusion The up-regulated expression of PRG-1 in cerebral cortex may be associated with the recurrent neonatal seizure-induced brain damage.

关 键 词：可塑性相关基因-1 惊厥 大脑皮质 发育 

分 类 号：R742.1[医药卫生—神经病学与精神病学]