α-L-岩藻糖苷酶对Lewis y抗原高表达卵巢癌细胞系的体外抑制作用  

Inhibitory Effect of α-L-fucosidase on Lewis y Antigen Overexpressed Human Ovarian Cancer Cells in vitro

作　　者：刘娟娟[1] 林蓓[1] 齐跃[1] 李飞飞[1] 郝莹莹[1] 刘大我[1] 赵越[1] 张帆[1] 朱连成[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2010年第5期321-324,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30170980;30571958;30872757);教育部博士点基金资助项目(20070159023)

摘　　要：目的探讨α-L-岩藻糖苷酶对Lewis y抗原高表达卵巢癌细胞系的体外增殖、黏附以及细胞膜通透性的影响。方法以α-L-岩藻糖苷酶处理α1,2-岩藻糖转移酶基因转染前、后卵巢癌细胞系作为实验组(RMG-I-H-A、RMG-I-A),以未经α-L-岩藻糖苷酶处理的细胞作为对照组(RMG-I-H-C、RMG-I-C)。利用免疫细胞化学染色法检测4组细胞中Lewis y抗原的表达,利用细胞计数法、荧光分光光度计测定细胞的生物学特性。结果α-L-岩藻糖苷酶处理后的实验组RMG-I-H-A和RMG-I-A细胞黏附性差,生长速度减慢,细胞边缘整齐,呈椭圆型,无延伸生长,处于细胞分裂期的细胞个数少。实验组RMG-I-H-A和RMG-I-A细胞的膜通透性比对照组RMG-I-H细胞增加了4.4倍,比RMG-I细胞增加了2.3倍。结论 Lewis y抗原具有促进卵巢癌细胞系增殖,提高其生存能力的作用。Objective To study the effect of α-L-fucosidase on proliferation,adhesion and the membrane permeability of human ovarian cancer cell line with the overexpression of Lewis y antigen in vitro. Methods The ovarian cancer cells transfected with the α1,2-fucosyltransfersae gene were treated with α-L-fucosidase （RMG-I-H-A,RMG-I-A）or without treatment of α-L-fucosidase as controls（RMG-I-C, RMG-I-H-C）. The expression of Lewis y antigen in the cells was detected by immunocytochemistry. The biological behaviors of the cells were analyzed by flow cytometry and fluorospectrophotometer. Results The RMG-I-H and RMG-I cells of experimental groups showed poor adhesive ability,slow growth rate,low proportion of the cells in mitotic phase,no extended growth,an oval/round appearance,a clear and even edge. The membrane permeability of the cells increased 4.4-fold and 2.3-fold compared with that of RMG-I-H-C and RMG-I-C cells,respectively. Conclusion The Lewis y antigen expression promotes the proliferation and the survival rate of ovarian carcinoma-derived RMG-I cells.

关 键 词：Α-L-岩藻糖苷酶 LEWIS y抗原 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]