贫营养湖泊花神湖和紫霞湖浮游细菌群落结构分析  

Analysis on bacterioplankton community structure in two oligotrophic lakes,Huashen Lake and Zixia Lake

作　　者：边园琦[1,2] 邢鹏[1] 李化炳[1,2] 吴庆龙[1] 

机构地区：[1]湖泊与环境国家重点实验室、中国科学院南京地理与湖泊研究所,南京210008 [2]中国科学院研究生院,北京100039

出　　处：《生态科学》2010年第2期109-115,共7页Ecological Science

基　　金：国家重点基础研究计划项目“湖泊蓝藻水华生态灾害形成机理及防治的基础研究”(资助项目号为2008CB418104);江苏省创新学者攀登项目(资助项目号为BK2009024);中国科学院南京地理与湖泊研究所领域前沿项目：沉水植物对湖泊浮游细菌群落结构的影响及其机制(08SL15004)

摘　　要：以南京市花神湖和紫霞湖两个贫营养型湖泊为研究对象，通过构建花神湖和紫霞湖16S rRNA基因克隆文库探讨了浮游细菌群落结构组成的变化。结果表明，花神湖和紫霞湖两湖泊水体中浮游细菌群落结构相似，主要隶属于放线菌门（Actinobacteria）、蓝藻门（Cvanobacteria）、α-变形菌门（Alphaproteobacteria）、β-变形菌门（Betaproteobacteria）、杆菌门（Bacteroidetes）、浮霉菌门（Planctomycetes）、疣微菌门（Verrucomicrobia）和芽单胞菌门（Gemmatimonadetes），其中放线菌门（Actinobacteria）、蓝藻门（Cyanobacteria）、β-变形菌门（Betaproteobacteria）是优势细菌类群。两个湖泊水体中75％的细菌与GenBank中已有的未培养细菌同源性高于97％，同时在两个克隆文库中还发现了6个淡水细菌新类群。通过对低纬度区域贫营养型湖泊浮游细菌群落结构的分析，加深了我们对浮游细菌多样性的了解，表明湖泊浮游细菌多样性有待进一步认识。In order to explore the composition of bacterioplankton community in oligotrophic lakes, clone libraries were constructed and analyzed for Huashen Lake and Zixia Lake located in the urban area of Nanjing City, respectively. Results showed that positive clones of Huashen Lake and Zixia Lake were mainly affiliated with Actinobacteria, Cyanobacteria, Alphaproteobacteria, Betaproteobacteria, Bacteroidetes, Planctomycetes, Verrucomicrobia and Gemmatimonadetes. About 75% of total bacterial clones obtained from Huashen lake and Zixia Lake were higher than 97% of homology with uncultured bacteria in GenBank. Meanwhile, six new freshwater bacterial clusters were found simultaneously in Huashen lake done library and Zixia lake clone library. The present study has broadened our knowledge on bacterioplankton diversity and suggested that the diversity of bacterioplankton in fxeshwater lakes still needs further study.

关 键 词：贫营养湖泊 浮游细菌 16S RRNA基因 克隆 

分 类 号：X172[环境科学与工程—环境科学] Q14[生物学—生态学]