增殖性糖尿病视网膜病变患者玻璃体中AngⅡ、VEGF的研究  

Study of angiotensin Ⅱ and vascular endothelial growth factor in the vitreous of patients with proliferative diabetic retinopathy

作　　者：丁菘[1] 

机构地区：[1]辽宁省鞍山市长大医院眼科,辽宁鞍山114005

出　　处：《中国医药导报》2010年第17期21-23,共3页China Medical Herald

摘　　要：目的:通过测定增殖性糖尿病视网膜病变(PDR)患者及非PDR患者玻璃体中AngⅡ、VEGF水平,探讨AngⅡ、VEGF在PDR发病中的作用,并分析两者的相关关系,为探讨PDR的发病机制提供实验依据。方法:采用放免法测定玻璃体及血浆中AngⅡ的水平,用酶联免疫吸附测定法测定玻璃体及血浆中VEGF的水平,用紫外分光光度法测定玻璃体及血清中ACE的水平。结果:①PDR患者玻璃体中AngⅡ、VEGF、ACE水平显著高于非PDR患者(P<0.01);PDR患者玻璃体中AngⅡ、VEGF、ACE水平显著高于血液中的水平(P<0.01)。②PDR患者玻璃体中AngⅡ水平与VEGF及ACE水平之间均呈正相关(P<0.01,P<0.05)。结论:①PDR患者玻璃体中AngⅡ、VEGF水平明显升高,推测AngⅡ、VEGF在PDR的病理过程中起一定作用;②PDR患者玻璃体中AngⅡ、ACE、VEGF水平均高于血液中的水平,推测眼内肾素-血管紧张素系统可能是独立的,PDR患者玻璃体中VEGF可能来源于眼内组织;③PDR患者玻璃体中AngⅡ水平与ACE、VEGF水平均呈正相关关系,提示高水平的AngⅡ可能由高水平的ACE所引起,AngⅡ、VEGF在PDR的发展中具有相辅相成、相互促进的作用。Objective：To explore the roles of AngⅡand VEGF in PDR by detecting the levels of them in vitreous of PDR patients and nonPDR patients,investigate the relationship between them and provide experimental basis and theoretical foundation for determining the pathogenesis of PDR.Methods：The level of AngⅡ in the vitreous and plasma was quantified by the radiommunoassay,the level of VEGF in the vitreous and plasma was quantified by the enzyme linked immunosorbent assay,and the level of ACE in the vitreous and serum was quantified by the ultraviolet-spectrophotometry.Results：①The levels of AngⅡ,VEGF and ACE of PDR patients in the vitreous were obviously higher than nonPDR patients（P0.01）.The levels of AngⅡ,VEGF and ACE of PDR patients in the vitreous were obviously higher than those in the blood（P0.01）.②There was a positive correlation between AngⅡ and VEGF in PDR patients in the vitreous.There was a positive correlation between AngⅡ and ACE in PDR patients in the vitreous（P0.01,P0.05）.Conclusion：①Elevated levels of AngⅡ and VEGF in PDR patients are considered that AngⅡ and VEGF play roles in the pathology course of PDR.②The levels of AngⅡ,ACE and VEGF in PDR patients in the vitreous are separately higher than those in the blood,which is considered that intraocular RAS system may be independent and VEGF may originate from intraocular organization.③The level of AngⅡ is positively correlated with that of ACE and VEGF in the vitreous of PDR patients,which indicates that high level of AngⅡ is caused by high level of ACE,AngⅡ and VEGF play interdependant roles in the onset and development of PDR.

关 键 词：增殖性糖尿病视网膜病变 血管紧张素Ⅱ 血管内皮生长因子 

分 类 号：R774.1[医药卫生—眼科]