Sunitinib对视网膜微血管内皮细胞增殖和迁移的影响  

Effects of Sunitinib on proliferation and migration of retinal microvascular endothelial cells

作　　者：侯培[1] 黄敏丽[1] 

机构地区：[1]广西医科大学第一附属医院眼科,广西南宁市530021

出　　处：《眼科新进展》2010年第6期508-511,516,共5页Recent Advances in Ophthalmology

基　　金：广西自然科学基金资助(编号:2010GXNSFA013140)~~

摘　　要：目的探讨苹果酸舒尼替尼(Sunitinib)对体外培养的恒河猴视网膜微血管内皮细胞(RF/6A)增殖和迁移及KDR mRNA表达的影响。方法选取不同浓度的Sunitinib作用于RF/6A,分别于培养24h和48h后采用SRB染色法观察不同浓度的Sunitinib对RF/6A增殖的影响;采用细胞划痕修复实验观察不同浓度的Sunitinib对RF/6A迁移的影响;同时利用RT-PCR法检测不同浓度的Sunitinib处理前后RF/6AKD RmRNA表达水平的变化。结果 Sunitinib对RF/6A的增殖有抑制作用且呈时间-剂量依赖性,0.00125mg·L-1、0.0025mg·L-1、0.005mg·L-1、0.01mg·L-1、0.02mg·L-1和0.04mg·L-1浓度的Sunitinib作用24h,对RF/6A增殖的抑制率分别为(12.009±0.038)%、(21.440±0.007)%、(35.434±0.015)%、(43.125±0.002)%、(53.700±0.001)%、(60.971±0.003)%,各组两两比较差异均有统计学意义(均为P<0.01);而48h后,其抑制率则分别为(36.872±0.006)%、(40.673±0.013)%、(47.313±0.004)%、(55.910±0.003)%、(63.120±0.003)%、(69.975±0.014)%,各组两两比较差异均有统计学意义(均为P<0.01)。培养基中加入0mg·L-1、0.0025mg·L-1、0.005mg·L-1、0.01mg·L-1、0.02mg·L-1浓度的Sunitinib24h后RF/6A的迁移距离分别为(203.3±2.2)μm、(145.4±4.4)μm、(123.9±2.6)μm、(96.1±3.1)μm、(46.6±2.9)μm。而48h后则为(313.1±4.1)μm、(213.9±2.8)μm、(193.9±4.2)μm、(134.5±3.2)μm、(109.9±5.7)μm。在同一时段,各组两两比较差异均有统计学意义(均为P<0.01)。0mg·L-1、0.0025mg·L-1、0.005mg·L-1、0.01mg·L-1、0.02mg·L-1浓度的Sunitinib作用24h后,RF/6AKDR mRNA的相对表达量分别为0.583±0.004、0.570±0.008、0.553±0.007、0.531±0.003、0.513±0.005。而48h后则分别为0.628±0.005、0.610±0.002、0.588±0.002、0.564±0.005、0.525±0.004。在同一时段,各组两两比较差异均有统计学意义(P<0.01)。结论 Sunitinib能够抑制视网膜微血管内皮细胞的增殖和迁移,其机制可能是通过下调VEGFR mRNA的表达来实现的。Objective To evaluate effects of Sunitinib on proliferation and migration of retinal microvascular endothelial cells （RF/6A） and expression of KDR mRNA in rhesus in vitro.Methods RF/6A cells were treated with different concentrations of Sunitinib.Effects of different concentrations of Sunitinib on proliferation of RF/6A were observed with SRB staining after being cultured for 24 hours and 48 hours.Effects of Sunitinib on migration of RF/6A were observed with cell wound-healing assay.Changes of RF/6A KDR mRNA levels before and after being treated with different concentrations of Sunitinib were observed by RT-PCR method.Results Sunitinib had inhibitory effect on RF/6A and with time-concentration dependent manner.The inhibitory rates on proliferation of RF/6A were（12.009±0.038）%,（21.440±0.007）%,（35.434±0.015）%,（43.125±0.002）%,（53.700±0.001）% and （60.971±0.003）%,respectively,after being treated with 0.001 25 mg·L-1,0.002 5 mg·L-1,0.005 mg·L-1,0.01 mg·L-1,0.02 mg·L-1 and 0.04 mg·L-1 Sunitinib for 24 hours,there were statistical differences between each two groups（all P0.01）.The inhibitory rates on proliferation of RF/6A were （36.872±0.006）%,（40.673±0.013）%,（47.313±0.004）%,（55.910±0.003）%,（63.120±0.003）% and （69.975±0.014）%,respectively,after being treated with 0.001 25 mg·L-1,0.002 5 mg·L-1,0.005 mg·L-1,0.01 mg·L-1,0.02 mg·L-1 and 0.04 mg·L-1 Sunitinib for 48 hours,there were statistical differences between each two groups （all P0.01）.The migration distances were （203.3±2.2）μm,（145.4±4.4）μm,（123.9±2.6）μm,（96.1±3.1）μm and （46.6±2.9）μm,respectively,after adding 0 mg·L-1,0.002 5 mg·L-1,0.005 mg·L-1,0.01 mg·L-1 and 0.02 mg·L-1 Sunitinib into culture medium for 24 hours,were （313.1±4.1）μm,（213.9±2.8）μm,（193.9±4.2）μm,（134.5±3.2）μm and （109.9±5.7）μm,respectively,after adding 0 mg·L-1,0.002 5 mg·L-1,0.005 mg·L-1,0.01 mg·L-1 and 0.02 mg·L-1 Sunitinib into culture medium for 4

关 键 词：苹果酸舒尼替尼 视网膜微血管内皮细胞 增殖 迁移 

分 类 号：Q959.836[生物学—动物学] R774.1[医药卫生—眼科]