大鼠乳腺癌CT灌注成像与肿瘤病理分级和微血管密度相关性研究  

Correlation of CT perfusion imaging of rat breast cancer with tumor pathological grade and microvascular density

作　　者：张辉[1] 邹利光[1] 逄鑫[1] 张松[1] 廖翠薇[1] 

机构地区：[1]第三军医大学新桥医院放射科,重庆400037

出　　处：《第三军医大学学报》2010年第11期1131-1135,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金(30770609);重庆市自然科学基金(CSTC2006BB5104)~~

摘　　要：目的研究诱发性大鼠乳腺癌CT灌注成像(CT perfusion imaging,CTPI)与肿瘤病理分级、微血管密度(microvascular vessel density,MVD)的相关性,探讨CTPI对乳腺癌的诊断价值。方法 Wistar雌性大鼠35只,7,12-二甲基苯蒽(7,12-dimethyl-1,2-benzanthracene,DMBA)灌胃诱发乳腺肿瘤。乳腺CT平扫和CTPI检查,经工作站后处理得到CTPI参数血流量(blood flow,BF)、血容量(blood volume,BV)、平均通过时间(mean transit time,MTT)、毛细血管通透性(permeability surface,PS),并计算时间密度曲线(time density curve,TDC)。处死大鼠后取乳腺肿瘤组织进行HE染色和Ⅷ因子相关抗原免疫组化染色,观察乳腺肿瘤病理类型和MVD。结果 30只大鼠成功诱发乳腺肿瘤,其中乳腺增生性疾病8只,乳腺癌22只。乳腺癌组与乳腺增生性疾病组的BF、BV分别为(151.72±93.43)vs(42.96±32.42)ml/(min.100g);(9.55±7.88)vs(2.17±1.36)ml/100g,乳腺癌显著高于乳腺增生性疾病(P<0.05)。乳腺癌中分化组和低分化组的BF、BV分别为(115.30±15.46)vs(256.52±132.19)ml/(min.100g);(9.76±9.00)vs(10.34±4.56)ml/100g,BF、BV在不同分化程度肿瘤之间有显著差异(P<0.05)。乳腺癌MTT为(2.43±1.64)s,显著低于乳腺增生性疾病(P<0.05),但在不同分化程度肿瘤之间无显著差异(P>0.05)。BF、BV、PS与MVD的相关性随组织分化程度的降低呈升高趋势,其相关系数分别为0.701、0.441和0.521,两者之间均呈显著正相关(P<0.05)。结论大鼠诱发性乳腺癌是CT灌注成像研究适合的模型,CT灌注成像有助于判断乳腺病变的良恶性、反映肿瘤组织分化程度,并与肿瘤微血管密度密切相关。Objective To study the correlation of CT perfusion imaging （ CTPI） of induced rat breast cancer with tumor pathological grade and microvascular density （ MVD） ,and the diagnostic value of CTPI for breast cancer. Methods Breast cancer was induced in 35 Wister male rats with intragastric 7,12-dimethyl-1,2-benzanthracene （ DMBA） treatment. Routine breast CT scanning and CTPI were performed. After processed at a workstation,CTPI parameters were obtained including blood flow （ BF） ,blood volume （ BV） ,mean transit time （ MTT） ,permeability surface （ PS） ,and time density curve （ TDC）. The rats were sacrificed and breast tumor tisue samples were collected. Breast tumor tisue samples were stained with HE and factor Ⅷ-related antigen immunohistochemistry to observe the pathological type and MVD of tumor. Results Breast tumor was successfully induced in 30 rats （ including 8 with breast hyperplasia and 22 with breast cancer）. The BF and BV were higher in rats with breast cancer than in those with breast hyperplasia 151. 72 ± 93. 43 vs 42. 96 ± 32. 42 ml/（ min.100 g） ,9. 55 ± 7. 88 vs 2. 17 ± 1. 36 ml/100 g,P 0. 05. The BF and BV were lower in patients with moderatelely-differentiated breast cancer than in those with poorly-differentiated breast cancer 115. 30 ±15. 46 vs 256. 52 ±132. 19 ml/（ min.100 g） ,9. 76 ±9. 00 vs 10. 34 ±4. 56 ml/（ min.100 g） ,P 0. 05. The MTT was significantly shorter in rats with breast cancer than in those with breast hyperplasia （ P 0. 05） ,but showed no significant difference in tumor differentiation （ P 0. 05）. The positive correlation of MVD with BF,BV and PS was increased with the decreased tumor differentiation with a correlation coefficient of 0. 701,0. 441 and 0. 521,respectively （ P 0. 05）. Conclusion Induced breast cancer in rats is a suitable model for CT perfusion study. CTPI can help to distinguish between benign and malignant breast diseases and show tumor differentiation,and is closely associated with MVD of tumo

关 键 词：体层摄影术 X线计算机 灌注成像 乳腺癌 血管生成 

分 类 号：R730.2[医药卫生—肿瘤] R730.44[医药卫生—临床医学]