血管抑素对角膜新生血管和VEGF表达的作用  

Effect of angiostatin on rat corneal neovas-cularizasion and the expression of vascular endothelial growth factor

作　　者：曾静[1] 罗殿中[1] 黄明汉[1] 邓妮妮[1] 党裔武[1] 

机构地区：[1]广西医科大学第一附属医院眼科,中国广西壮族自治区南宁市530021

出　　处：《国际眼科杂志》2010年第6期1052-1055,共4页International Eye Science

基　　金：中国广西壮族自治区自然科学基金资助项目(No.0728131);中国广西壮族自治区教育厅研究生科研创新基金资助项目(No.2008105981001D24)~~

摘　　要：目的:探讨血管抑素(angiostatin,AS)对鼠碱烧伤角膜新生血管(corneal neovascularization,CNV)及血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)表达的作用,探讨 AS对CNV的作用及机制。方法:SD大鼠105只随机分为正常对照组(5只)、生理盐水组、地塞米松组、AS1组、AS2组(各25只),除正常组外,实验各组制作左眼碱烧伤CNV模型,分别予生理盐水、1g/L地塞米松液、10μg/mLAS液、20μg/mLAS液点眼,4次/d,于碱烧伤后1,3,7,14,21d运用裂隙灯、免疫组织化学方法观察新生血管长度及面积、VEGF的表达。结果:AS治疗组碱烧伤后第3d起各时间点CNV面积均明显小于盐水组(P<0.05)。碱烧伤后各时间点AS治疗组VEGF表达量均明显少于盐水组(P<0.05)。结论:AS能显著抑制碱烧伤CNV,可能与其抑制VEGF的表达有关。AIM：To investigate the anti-angiogenesis effect of angiostatin（AS） on rat corneal neovascularization（CNV） and the expression of vascular endothelial growth factor（VEGF）.·METHODS： CNV models were established in 105 SD rats,which were randomly divided into normal group（5 SD rats） and experimental group（100 SD rats,induced by alkali burn）：normal saline group, 1g/L dexamethasone group, and different concentration angiostatin-treated group（group AS1 10μg/mL and group AS2 20μg/mL）. Slit-lamp microscope was performed and the area of CNV was calculated on 3, 7, 14, 21 day after injury. Then 5 rats were randomly sacrificed and the corneas were taken for histopathological and immunohistochemistry examina-trion.·RESULTS： The area of CNV in the AS groups was smaller than that in the normal saline group 3 days after cautery（P0.05）. The expression of VEGF had statisti-cally significant difference between AS group and normal saline group（P0.05）.·CONCLUSION： AS can effectively inhibit rat CNV after alkali burn. The mechanism probably is down-regulation of the expression of VEGF.

关 键 词：碱烧伤 角膜新生血管 血管抑素 血管内皮生长因子 

分 类 号：R779.1[医药卫生—眼科]