少孢节丛孢菌胞外蛋白酶的诱导与生化性质  

Induction and biochemical characteristics of extracellular protease from Arthrobotrys oligospora

作　　者：王瑞[1] 杨莲茹[1] 杨晓野[1] 马亚囡 赵治国[1] 赵晓慧[1] 李文生[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古神元生物工程股份有限公司,内蒙古呼和浩特010010

出　　处：《中国兽医科学》2010年第5期446-451,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30260081);内蒙古自然科学基金项目(20080404MS0410)

摘　　要：应用不同诱导剂和不同营养组成的液体培养基诱导培养捕食线虫性真菌——少孢节丛孢菌产生发酵液,经浓缩处理后,对发酵液中的蛋白质含量、酶活性以及相关的生化性质进行了测定。结果表明,培养基的组成成分和相关诱导剂能够显著影响少孢节丛孢菌的代谢过程;而且秀丽新杆线虫刺激少孢节丛孢菌产生的培养基滤液和氨基酸刺激所产生的发酵液内含成分不同;蛋白酶活性越高的滤液中,磷酸酶活性越低;产生胞外蛋白酶的最佳培养时间为6d。Different liquid medium and inductors were used to produce fermentation broth of the nematode-trapping fungus Arthrobotrys oligospora.The protein concentration,enzyme activity and related biochemical properties of the concentrated broth were determined.Results showed the method could significantly influence metabolic process of A.oligospora,and the composition of the broth filtrate induced by Caenorhabditis elegans was different from that by amino acid.Besides,the broth filtrate with high protease activity had low phosphatase activity.The extracellular protease could be gotten after A.oligospora was cultured for 6 days.

关 键 词：捕食线虫性真菌 少孢节丛孢菌 胞外蛋白酶 磷酸酶 

分 类 号：S852.66[农业科学—基础兽医学]