姜黄素阻断NF-κB信号通路抑制人卵巢癌细胞株3AO增殖  

Effects of curcumin on proliferation of human ovarian cancer cell line 3AO in vitro

作　　者：李帆[1] 马向东[1] 陈必良[1] 辛晓燕[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710032

出　　处：《现代肿瘤医学》2010年第6期1077-1080,共4页Journal of Modern Oncology

摘　　要：目的:探讨姜黄素(curcumin)对体外培养人卵巢癌细胞株3AO细胞增殖抑制及其作用机制。方法:选用人卵巢癌细胞株3AO进行体外培养,以20μg/L、40μg/L、80μg/L姜黄素分别处理3AO细胞24-48h,MTT比色法检测细胞生长活性,流式细胞仪检测细胞周期时相变化,Western blot法检测NF-κB、p-NF-κB、IκB、p-IκB蛋白的变化。结果:姜黄素对人卵巢癌细胞株3AO生长的抑制作用,呈剂量、时间依赖性。流式细胞仪分析证实姜黄素能使3AO细胞周期阻滞于G0/G1期,Western blot结果显示随姜黄素作用浓度的增加,NF-κB、p-NF-κB蛋白表达水平明显下降,IκB、p-IκB蛋白表达没有明显改变。结论:姜黄素对3AO细胞有明显的增殖抑制作用,此作用可以通过下调NF-κB蛋白表达及磷酸化实现,这是姜黄素抑制卵巢癌细胞生长的分子机制之一。Objective： To investigate the effects of curcumin on proliferation of human ovarian cancer cell line 3 AO in vitro. Methods：Human ovarian cancer cell line 3AO was cultured in vitro and treated with curcumin of different concentration （20ug/L ,40ug/L, 80ug/L）. The inhibition rates of cells were detected by MTT assay; cell cycle distribution was detected by/low cytometry（FCM） ; expression of NF - kB,p - NF - kB ,IKB,p - IkB in 3AO cell was observed by western bolt. Results ：Curcumin inhibited the proliferation of 3AO cells in a dose -and time -dependent manner. Cell growth was arrested at G0/G1 stage. The expression of NF - kB, p - NF - kB was increased in a dose dependent manner,but the expression of IkB,p- IkB was not affected. Conclusion： Curcumin can inhibits the pro- liferation of 3AO,it is related to the down -regulation of NF- kB and p- NF- kB.

关 键 词：姜黄素 卵巢癌 核转录因子ΚB 增殖 

分 类 号：R73-361[医药卫生—肿瘤] R737.31[医药卫生—临床医学]