γ-IFN对于兔盆腔常规分割照射后输尿管放射性损伤及纤维化的影响  

Efect of γ-IFN to the radiation damage and radiation fibrotic of rabbit ureter after cavitas pelvisrute fractionated irradiation

作　　者：杨蕴一[1] 王娟[1] 张丽[1] 赵恒芳[1] 代丽[1] 刘孜[1] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061

出　　处：《现代肿瘤医学》2010年第6期1080-1082,共3页Journal of Modern Oncology

基　　金：陕西省科技攻关项目(2005K12-G5-4)

摘　　要：目的:探讨γ-IFN对兔盆腔常规分割照射后输尿管放射性损伤及纤维化的影响。方法:建立兔放射性输尿管损伤的动物模型,52只新西兰兔随机分为常规照射组、γ-IFN组及正常对照组,除正常对照组外其余兔盆腔均接受6MV-X线照射。γ-IFN组从照射后第5天给予γ-IFN250,000U·kg-1,肌注每周1次,共5次。常规照射组给予5ml·kg-1的生理盐水。照射后4周,8周,12周,16周处死实验兔,取其照射范围内的输尿管组织。用HE染色,原位杂交染色及免疫组化方法分析γ-IFN对于放射性输尿管损伤及纤维化影响。结果:HE染色观察放射线导致输尿管损伤的组织结构变化,γ-IFN组与单纯照射组比较,照射后4,8,12周其损伤程度均有显著性差异(P<0.05),16周则无显著性差异(P>0.05);TGF-β1 mRNA原位杂交实验,γ-IFN组输尿管黏膜的TGF-β1 mRNA表达在照射后4,8,12,16周低于常规照射组,有显著性差异(P<0.05),与正常对照组比较也有显著性差异(P<0.05);Ⅲ型胶原免疫组化结果显示γ-IFN组与单纯照射组均存在显著性差异(P<0.05),较正常对照组差异有显著性(P<0.05)。结论:γ-IFN能够减轻实验兔放射性输尿管损伤,抑制放射后输尿管黏膜TGF-β1 mRNA的升高,降低Ⅲ型胶原的表达,减轻放射性输尿管损伤及纤维化的形成。Objective： To investigate the efect of 3＇ - IFN to the radiation damage and radiation fibrotic of the rabbit ureter after cavitas pelvisrute fraetionated irradiation. Methods： To establish rabbit radioactive ureteral injury animal models, 52 New Zealand rabbits were divided into conventional radiation group, 3＇ -IFN group, and normal con- trois, except the normal control group, all the rabbit were exposured pelvic cavity by 6MVX ,after 5 days exposure of 3＇ -IFN group, treated by 250,000U 3＇ -IFN , once a week, 5ml sodium chloride to illuminate conventional radiation group. After four weeks, 8 weeks,12 weeks,16 weeks,executed rabbits,take the ureter in the exposure scope. HE dyeing, situ hyb - ridization and immunohistoehemieal staining method were used to analyze radioactive ureteral injury and fibrosis. Results： HE dyeing, after 4,8,12 weeks , radioactive ureteral injury and fibrosis of the con - ventional radiation group and 3＇ -IFN group were significantly different （P 〈 0.05 ） , 16 weeks without significant difference （ P 〉 0.05 ）. TGFI31 mRNA of the situ hybridization , at 4,8,12,16 weeks after irradiationin conventional radiation group and 3＇ - IFN group had significant difference （ P 〈 0. 05 ）, and were significantly different compared to normal controls （ P 〈 0.05 ） . The COLIII immunohistoehemieal results in conventional radiation group and 3＇ - IFN group,normal controls had significant difference （ P 〈 0.05 ）. Conclusion： 3＇ - IFN relieved radioactive ureteral injury, suppressed the step up of the TGFIS1 mRNA in tunica mucosa ureteris , reduced COLIII expression , reduced radiation damage and radiation fibrotic of the rabbit ureter.

关 键 词：Γ-IFN 放射性输尿管损伤 TGF-Β1 mRNA Ⅲ型胶原 兔 

分 类 号：R73-362[医药卫生—肿瘤] R737.13[医药卫生—临床医学]