IGF-2基因印迹状态与临床病理因素的关系  

Clinical significance of IGF-2 imprinting status in gastric cancer

作　　者：陈腾[1] 左青松[1] 赵荣华 奉典旭[1] 陈超[1] 蒋一鸣[1] 韩峰[1] 

机构地区：[1]上海中医药大学附属普陀医院普外科,上海200062 [2]加拿大Saskatchewan大学医学院皇家医院外科

出　　处：《外科理论与实践》2010年第3期245-248,共4页Journal of Surgery Concepts & Practice

基　　金：上海市科委局管科技基金项目(054006)

摘　　要：目的:探讨胃癌组织中,IGF-2(insulin-like growth factors2)基因的印迹状态及其与各临床病理因素间的关系。方法:应用PCR、RT-PCR及限制性内切酶(ApaⅠ)酶切等方法,检测33例胃癌组织中IGF-2基因的印迹状态,分析IGF-2基因印迹丧失(loss of imprinting,LOI)及其与胃癌各临床病理因素之间的关系。结果:胃癌组织中,IGF-2基因印迹的丧失率为48.5%(16/33),癌近旁黏膜的IGF-2基因印迹丧失率为21%(7/33),癌远旁黏膜的丧失率为12.1%(4/33)。同时,在有淋巴结转移的胃癌病例中IGF-2基因印迹丧失率(60.9%)明显高于无淋巴结转移者(20.0%);TNM分期Ⅲ、Ⅳ期的胃癌IGF-2基因印迹丧失率(60.0%)明显高于Ⅰ、Ⅱ期者(12.5%)(P均<0.05)。结论:IGF-2基因印迹丧失是贯穿于胃癌发生、发展全过程的表观遗传异常,其与胃癌的临床、病理分期及病人预后相关。Objective To investigate the IGF-2 imprinting status in tissues of gastric cancerpatients and its relationship with the other clinical / pathological parameters in gastric cancer. Methods IGF-2 imprinting status was determined by PCR and RT-PCR, followed by restrictive endonuclease （Apa I） digestion in 90 patients with gastric adenocarcinoma. The loss of imprinting （LOI） of IGF-2, and its relationship with the other clinicopathologic parameters was studied in a group of prospectively recruited patients. Results LOI was positive in 16 of 33 cases（48.5%） of gastric cancer tissue; 7 of 33（21.2%） in adjacent neighboring mucosa; and 4 of 33（12.1%）） in distant neighboring mucosa. The prevalence of IGF-2 LOI in cancer was significantly higher in patients with positive lymph node metastasis （60.7%） and in cases belonging to advanced stages （TNM： Ⅲ/Ⅳ） （93.7%）, as compare to those with negative lymph-nodes （20.0%） （P0.05）, and those belonging to early stages （TNM： Ⅰ/Ⅱ） （12.5%）（P0.05）, respectively. Conclusions IGF-2 LOI has been confirmed to be associated with the initiation and development of gastric cancer, which was related with clinical staging; pathological staging and prognosis.

关 键 词：胃癌 IGF-2 基因印迹 

分 类 号：R735.2[医药卫生—肿瘤]