构建RNA干扰真核表达载体靶向抑制乳腺癌细胞株MDA-MB-231内的VEGF-C表达之研究  

To construct the RNA interference eukaryotic expression vector targeting VEGF-C in the human breast cancer cell line aiming at reducing its invasiveness

作　　者：钟强[1] 朱晨芳[1] 戚晓亮[1] 孙波[1] 顾岩[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院普外科,上海200011

出　　处：《外科理论与实践》2010年第3期263-268,共6页Journal of Surgery Concepts & Practice

基　　金：上海市科学技术委员会科研计划项目(06DZ19506);国家自然基金(30872521)

摘　　要：目的:通过构建RNA干扰真核表达载体人乳腺癌细胞株MDA-MB-231内VEGF-C后的生物学变化。方法:构建pGCsi-VEGF-C真核表达载体,建立VEGF-C稳定低表达的MDA-MB-231人乳腺癌细胞株,检测该细胞株的体外增殖和侵袭能力及体内移植瘤生长和淋巴结转移的变化。结果:成功构建了pGCsi-VEGF-C真核表达载体,沉默效率达90%;筛选并获得VEGF-C稳定低表达的MDA-MB-231细胞株,该细胞株的体外增殖及侵袭能力,分别下降40%和35%,体内移植瘤的生长减慢和癌细胞淋巴结转移数量明显下降(P<0.05)。结论:VEGF-C的表达量下降可显著降低MDA-MB-231细胞株的增殖、侵袭及淋巴结转移能力。Objective To construct RNA interference the eukaryotic expression vector targetting the vascular endothelial growth factor-C （VEGF-C） in the human breastcancer cell line MDA-MB-231 aiming at reducing its invasiveness. Methods To construct and select pGCsi-VEGF-C as the eukaryotic expression vector, and to establish a new human breast cancer cell line which offers a stable low-VEGF-C expression; and to detect the resulting changes of cell proliferation and cell invasveness in vitro and xenograft growth and lymph node metastasis in vivo. Results We arrived at successfully constructing the pGCsi-VEGF-C eukaryotic expression vector, its silence efficiency was up to 90%; we also obtained the low stability of VEGF-C expression in the MDA-MB-231 cell line, its invasiveness was reduced in vitro（P0.05） by 40% and 35% respectively; its xenograft growth rate slowed and the number of lymph node metastasis significantly decreased in vivo. Conclusions The suppression of VEGF-C can significantly reduce the rates of proliferation, invasiveness and lymph node metastasis of the MDA-MB-231 cell line.

关 键 词：VEGF-C 乳腺癌 RNA干扰 真核表达载体 

分 类 号：R737.9[医药卫生—肿瘤]