利用SSR标记鉴定甘蓝型油菜秦优11号种子纯度  

Purity Identification of Brassica Qinyou No.11 Using SSR Markers

作　　者：宋贤勇[1] 包月红[1] 杨俊涛[1] 管荣展[2] 刘桂超[1] 

机构地区：[1]江苏中江种业股份有限公司,南京210036 [2]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《分子植物育种》2010年第3期497-500,共4页Molecular Plant Breeding

摘　　要：本研究利用SSR分子标记技术,对甘蓝型杂交油菜秦优11号的双亲及F1之间的多态性进行了引物筛选和纯度检测技术研究。结果表明,在已筛选的150对引物中,有2对引物(BN12和BN1)表现为稳定的共显性,即杂种表现为父母本互补的带型。利用这2对引物对4四个杂交油菜样品秦优11号进行纯度鉴定,结果分别为79.1%、81.6%、85.5%和86.7%,对应样品田间正季鉴定结果为83.0%、84.8%、87.8%和89.5%,样本株数为200时,误差均在容许误差范围内,这表明筛选的SSR分子标记可以用于秦优11号杂交种纯度的快速、准确鉴定。In this paper,we conducted primers screening and purity identification of both parents and F1 of Brassica（Brassica napus L.） Qinyou No.11 by using SSR molecular marker technique.The results showed that there were 2 pairs primers,BN12 and BN1,presented stable codominance among 150 pairs of screened SSR primers,in which the banding pattern of hybrid seed was complementary types of their parents.The purity for four hybrid seed sample of Qinyou No.11 were 79.1%,81.6%,85.5% and 86.7%,respectively,by employing these two pairs of primers.The field identification results of corresponding samples were 83.0%,84.8%,87.8% and 89.5%,respectively.The deviation were between allowable error when the sample reached to 200,which indicated that SSR markers（BN12 and BN1） could be used for rapid and accurate identification of seed purity of Qinyou No.11.

关 键 词：甘蓝型杂交油菜 秦优11号 育性转换 SSR标记 

分 类 号：S565.4[农业科学—作物学]