甘薯淀粉含量的QTL定位  

Mapping QTLs for Starch Content in Sweetpotato

作　　者：李爱贤[1,2] 刘庆昌[1] 王庆美[2] 翟红[1] 阎文昭[3] 张海燕[2] 李明[3] 

机构地区：[1]中国农业大学农业部作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,教育部作物杂种优势研究与利用重点实验室,北京100193 [2]山东省农业科学院作物研究所,济南250100 [3]四川省农业科学院生物技术核技术研究所,成都610066

出　　处：《分子植物育种》2010年第3期516-520,共5页Molecular Plant Breeding

基　　金：国家甘薯产业技术体系;国家863计划(2006AA100107);国家科技支撑计划(2006BAD01A06);农业部948计划(2006-G21-02);农业部公益性行业科技(nyhyzx07-012-03);山东省农科院青年基金(2007YQN004)共同资助

摘　　要：本研究以高淀粉甘薯品种漯徐薯8号(母本)和低淀粉甘薯品种郑薯20(父本)杂交得到的F1分离群体,选择其中240个单株为材料,以该群体所构建的分子连锁图谱为基础,以2008年和2009年所测定的淀粉含量为指标,采用复合区间作图法并利用QTLMapper2.0软件分析了甘薯淀粉含量的QTL。实验结果表明,检测到了1个主效位点,位于父本郑薯20的Z31连锁群上a02b40.02ds**和a08b37.03fs*两个标记之间,QTL的加性效应为-0.73,解释表型变异的7.70%。定位甘薯淀粉含量相关的QTL,将为发掘与淀粉含量相关的基因奠定基础。In this paper,we employed 240 individuals of F1 population,which derived from a cross between sweet- potato cv.‘Luoxushu 8’,a high starch content cultivar,and cv.‘Zhengshu 20’,a low starch content cultivar as ex- perimental materials,Others,based on the molecular linkage map constructed with SRAP markers using the F1 population,the QTL Mapper 2.0 and the composite interval mapping were employed to identify quantitative traits loci （QTL） associated with starch content of sweetpotato in 2008 and 2009,QTL analysis revealed the presence of one region having significant effect on the variation of starch content in Zhengshu 20 map. The region was associated with markers a02b40.02ds＊＊ and a08b37.03fs＊ in the linkage group Z31,its additive effect was -0.73,and it could be used to explain 7.70% of the total variation of the starch content. Mapping QTLs for starch content will provide a powerful tool to explore genes related to starch content in sweetpotato.

关 键 词：甘薯(Ipomoea batatas(L.)Lam.) 淀粉含量 QTL定位 

分 类 号：S531[农业科学—作物学]