骨髓基质细胞在气电纺丝蛋白纤维支架上早期粘附的研究  

Early Adhesion of Bone Marrow Sroma Cells on Air/jet Electrospinning Silk Fibroin Nanofiber Scaffolds

作　　者：李罡[1] 姚勇毅[2] 李小玉[3] 李艳红[1] 

机构地区：[1]昆明医学院口腔医学系,云南昆明650031 [2]四川大学纺织工程学院,四川成都610041 [3]四川大学华西口腔医学院,四川成都610041

出　　处：《昆明医学院学报》2010年第5期48-52,共5页Journal of Kunming Medical College

摘　　要：目的检测大鼠骨髓基质细胞在气电纺蚕丝蛋白纳米纤维支架上的早期粘附状况,研究其基本生物学性能.方法将具有良好生物性能的蚕丝蛋白通过气流高压静电纺丝工艺制成纳米纤维,并且通过激光共聚焦显微镜对骨髓基质细胞在气电纺蚕丝蛋白纳米纤维支架表面的早期粘附进行观察.结果骨髓基质细胞对气电纺蚕丝蛋白无纺织物表现出良好的亲和性,细胞在材料表面4h即形成了稳定的粘附,并有部分细胞开始在材料表面铺展,铺展细胞数与粘附细胞数的比率为75.9%,而对照组仅为49.63%.结论气电纺蚕丝蛋白纳米纤维支架具有良好的亲和性,提示其作为骨组织工程材料的可能性.Objective To study the biocompatibility of air/jet electrospinning silk fibroin nanofiber scaffolds by detecting the early adhesion of rat bone marrow stroma cells on the scaffolds.Methods In this study,the silk fibroin nanofiber was produced by air/jet Electrospinning,and the early adhesion of bone marrow stroma cells on the nanofiber scaffolds was observed by laser scanning confocal microscope.Results Bone marrow stroma cells had good affinity on air/jet electrospinning silk fibroin nanofiber scaffolds.The cells adhered on the surface of nanofiber firmly within 4 h,and there were some cells spreading on the scaffolds.The ratio between spreaded cells and adhered cells was 75.9%,while the ratio was only 49.63% in control group. Conclusion Air/jet electrospinning silk fibroin nanofiber scaffolds have good biocompatibility,and may be a good candidate for the biomedical applications in the bone tissue engineering.

关 键 词：蚕丝 高压静电纺丝 骨髓基质细胞 

分 类 号：R783.1[医药卫生—口腔医学]