大鼠精神分裂症后自发性运动量及海马神经发生的改变  

Changes of locomotor activities and hippocampal neurogenesis in rats subject to schizophrenia

作　　者：刘娟[1,2] 韩怀钦[1] 文玉军[1] 王银[1] 张义伟[1] 刘印明[3] 马江波[1] 

机构地区：[1]宁夏医科大学基础医学院人体解剖学教研室 [2]生育力保持省部共建教育部重点实验室,银川750004 [3]日本顺天堂大学医学部精神医学讲座,东京1138421

出　　处：《神经解剖学杂志》2010年第3期314-318,共5页Chinese Journal of Neuroanatomy

基　　金：宁夏教育厅重点项目;宁夏医科大学特殊人才科研启动项目(XT200815)资助

摘　　要：目的:探讨成年大鼠精神分裂症后自发性运动量和海马神经发生的改变。方法:通过连续2周腹腔注射环苯已哌啶(phencyclidine,PCP)建立大鼠精神分裂症模型,利用动物运动分析系统监测大鼠自发性运动量,5-溴-2-脱氧尿苷嘧啶(BrdU)标记新生的神经细胞,用免疫荧光标记法监测海马齿状回BrdU、NeuN、S-100β的表达,利用激光共聚焦显微镜观察海马神经细胞的增殖与分化情况。结果:精神分裂症模型大鼠比对照组大鼠自发性运动量高出2～3倍(P<0.05);BrdU阳性细胞数约下降了24%(P<0.05);两组BrdU阳性细胞的分化无明显差异性(P>0.05),大多分化为神经元。结论:精神分裂症可导致成年大鼠自发性运动量增加,并引起海马神经发生的改变,降低神经细胞的增殖。Objective： To investigate the changes of counts of locomotor activities and hippocampal neurogenesis in adult rats subject to schizophrenic. Methods： Rats received intraperitoneal injection of phencyclidine （PCP） daily for 14 consecutive days for schizophrenic model. Locomotor activities were recorded using a Supermex behavior analyzing system. The newly generated neurocytes were labeled by bromodeoxyuridine （BrdU）. The expression of neuronal nuclei （NeuN）. S-100β and the number of BrdU positive cells in hippocampal dentate gyrus were detected by immunofluorescence histochemical staining. Results： The counts of locomotor activities in schizophrenic model rats were increased 2- to 3-fold compared to that of control group（P 〈 0. 05） and displayed a significant reduction by 24% in BrdU-positive cells compared with that of control group （ P 〈 0. 05 ）. No significant difference in the differentiation of BrdU-positive cells were noted （P 〉 0.05 ） and most of BrdU-positive cells differentiated into neurons in both groups. Conclusion： The schizo- phrenic could cause counts of locomotor activities increase and lead to changes of hippocampal neurogenesis and inhibit proliferations of neurons of adult rats.

关 键 词：精神分裂症 环苯已哌啶 海马神经发生 自发性运动 大鼠 

分 类 号：R749.3[医药卫生—神经病学与精神病学]