胰岛素对局灶脑缺血c-jun基因表达的影响  

The effect of insulin on c jun gene expression in the rat brain with focal ischemia.

作　　者：王艺东[1,2,1,3] 黄如训[1,2,1,3] 曾木圣 李玲[1,2,1,3] 陶玉倩[1,2,1,3] 

机构地区：[1]中山医科大学附属第一医院神经科 [2]中山医科大学孙逸仙纪念医院神经科 [3]中山医科大学肿瘤研究所

出　　处：《中国神经精神疾病杂志》1999年第1期21-22,共2页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的观察不同剂量胰岛素对高血压大鼠局灶缺血脑组织ｃｊｕｎ基因表达的影响。方法以肾血管性高血压大鼠（ＲＨＲ）复制大脑中动脉闭塞（ＭＣＡＯ）模型，采用原位杂交技术检测不同剂量胰岛素组和对照组ＭＣＡＯ后３ｈ脑组织ｃｊｕｎ基因的表达。结果与对照组相比，在缺血侧广泛的大脑皮层，低剂量胰岛素组ｃｊｕｎｍＲＡＮ有统计学意义的增加（Ｐ＜００５），而较高剂量胰岛素组ｃｊｕｎｍＲＮＡ增加更为显著（Ｐ＜００１）。结论胰岛素促进缺血脑组织ｃｊｕｎ基因表达可能是其神经保护作用机制之一，而且这种作用呈剂量依赖性。Objective To investigate the effect of insulin on c jun gene expression following focal cerebral ischemia in hypertensive rats.Methods The renovascular hypertensive rats(RHR)were divided into 4 groups receiving either insulin only (Group A),insulin plus glucose(Group B and C,dose differently) or normal saline (Group D)immediately after middle cerebral artery occlusion (MCAO).The level of c jun mRNA was measured by situ hybridization at 3h after MCAO. Results Low or high dose of insulin all increased c jun expression in ipsilateral cortex following MCAO and c jun mRAN increased along with elevated insulin dose.Conclusions These results indicate that the effect of insulin on c jun expression may be one of the mechanisms of neuroprotection of insulin,which is dose dependent.

关 键 词：胰岛素 脑缺血 C-JUN 基因表达 

分 类 号：R743.31[医药卫生—神经病学与精神病学]