难治性癫痫患者交感神经-β肾上腺素能受体测定  

The study on the density of β-adrenoreceptor of sympathetic nervous system in lymphocytes from Intractable epilepsy patients

作　　者：白婧[1] 李亚军[1] 张蓓[1] 王敏娟[1] 郭长江[1] 石少亭[1] 折潇[1] 

机构地区：[1]西安医学院附属医院神经内科,西安710077

出　　处：《中国神经精神疾病杂志》2010年第5期313-315,共3页Chinese Journal of Nervous and Mental Diseases

基　　金：西安医学院重点学科建设专项资金资助项目(编号:2009-02)

摘　　要：目的探讨难治性癫痫患者发作间期交感神经β肾上腺素能系统的特征及美托洛尔对其的影响。方法对33例难治性癫痫患者和30名健康对照者采用放射性配基(3H-DHA)结合分析法测定外周血淋巴细胞β受体密度(βmax);用放射免疫法测定外周血淋巴细胞内环核苷酸(cyclic adenosine monophosphate,cAMP)含量;血浆去甲肾上腺素(noradrenalin,NE)含量的测定采用高效液相-电化学法。对难治性癫痫患者用美托洛尔治疗8周后再次测定上述指标并进行比较。结果与正常对照组比较,癫痫患者中病史<5年者外周血淋巴细胞β受体密度、cAMP、血浆NE浓度含量比较分别为:(271±96)fmol/107cell、(1.87±0.21)pmol/mg pro、(1.82±0.18)pmol/L(P<0.05);病史>5年者外周血淋巴细胞β受体密度、cAMP、血浆NE浓度含量比较分别为:(208±96)fmol/107cell、(1.73±0.19)pmol/mg pro、(1.93±0.15)pmol/L(P<0.01)。美托洛尔治疗8周后外周血淋巴细胞β受体密度、cAMP含量上调,血浆NE浓度明显降低。结论交感神经β肾上腺素能系统参与了癫痫发作致心脏损害的病理生理过程,美托洛尔干预处理能改善心肌细胞的信息传导,有助于损伤的心肌细胞生物学功能恢复。Objective To investigate the density of β-adrenergic system of sympathetic nervous system in lymphocytes in intractable epilepsy patients during the interictal period and whether metroprolol can influence the intractable epilepsy-induced changes in lymphocyte β-adrenoreceptor density.Methods Thirty-three patients with intractable epilepsy and 30 health controls were included in the present study.The density of β-adrenoreceptor,the cyclic nucleotide（cAMP）contents of peripheral lymphocyte and the plasma norepinephrine（NE）were examined using radioligand binding assay（3H-DHA）,radioimmunity assay and HPLC combined with electrochemical detection before and eight weeks after metoprolol therapy,respectively.Results The concentrations of β-adrenoceptor（271±96 fmol/107cell）in peripheral lymphocyte,the cyclic nucleotide contents（1.87±0.21 pmol/mg pro）and the plasma norepinephrine（1.82±0.18 pmol/L）in patients with less than five year history of intractable epilepsy were significantly higher that those in controls（all P0.05）;Similarly,The concentrations of β-adrenoceptor（208±96 fmol/107cell）in peripheral lymphocyte,the cyclic nucleotide contents（1.73±0.19 pmol/mg pro）and the plasma norepinephrine（1.93±0.15 pmol/L）in patients with greater than five year history of intractable epilepsy were significantly higher that those in controls（all P0.01）.Treatment with metoprolol for 8 weeks significantly reversed epilepsy-induced changes in above parameters.Conclusions β-adrenergic system of sympathetic nervous system participates in the pathophysiological process of seizure-induced heart damage.Metoprolol can improve the signal transduction of cardiac myocytes and enhance the functional recovery of of injured cardiac myocytes.

关 键 词：癫痫 交感神经 肾上腺素能受体 Β受体阻滞剂 

分 类 号：R742.1[医药卫生—神经病学与精神病学]