前列腺癌DNA倍体、PCNA、iMVD免疫组化研究  

DNA ploidy、PCNA and iMVD in prostatic carcinoma

作　　者：李金庆[1,2,3] 项锋钢[1,2,3] 董绍萃[1,2,3] 牛兆山[1,2,3] 马卫海[1,2,3] 申东亮[1,2,3] 

机构地区：[1]青岛医学院第二附属医院泌尿外科 [2]青岛医学院病理解剖教研室 [3]青岛医学院附属医院泌尿外科

出　　处：《中华泌尿外科杂志》1999年第1期36-38,共3页Chinese Journal of Urology

摘　　要：目的探讨前列腺癌ＤＮＡ倍体、增殖细胞核抗原（ＰＣＮＡ）、微血管密度（ｉＭＶＤ）与癌分级及预后的关系。方法应用计算机图像分析技术、免疫组织化学（ＬＳＡＢ）法，测定３０例前列腺癌（ＰＣ）、２０例前列腺增生症（ＢＰＨ）细胞核ＤＮＡ倍体、ＰＣＮＡ阳性指数、ｉＭＶＤ的变化。结果随癌分化程度降低，ＤＮＡ倍体增加、ＰＣＮＡ阳性指数升高、ｉＭＶＤ增加，其差异有显著性（Ｐ＜００５或Ｐ＜００１）；２年内死亡者，其ＤＮＡ倍体、ＰＣＮＡ阳性指数、ｉＭＶＤ均高于存活２年以上者（均为Ｐ＜０．００１）。结论ｉＭＶＤ较癌分级更准确地反映ＰＣ的预后。ＤＮＡ倍体、ＰＣＮＡ阳性指数。Objective To determine the relationship between these biologic markers and tumor grade and prognosis of the carcinoma. Metheds 30 cases of prostatic carcinoma (PC) and 20 cases of benign prostatic hyperplasia (BPH) were studied on DNA ploidy proliferating cell nuclear antigen (PCNA) and intratumoral microvascular density (iMVD) with computer assisted image analysis and immunohistochemistry (LSAB). Results The DNA ploidy,PCNA labeling index and iMVD were increased along with decreasing of the tumor histological grading.The DNA ploidy was significantly different among PC 3、PC 2、PC 1 and BPH ( P <0.05 or P < 0.01), so were the PCNA labeling index and iMVD ( P <0.05 or P < 0.01). The DNA ploidy、PCNA labeling index and iMVD were higher in patients with prostatic carcinoma died within two years than those survived for over two years ( P <0.001). Conclusions iMVD is a more accurate predictor than tumor grading in the evaluation of the prognosis of prostatic carcinoma. DNA ploidy, PCNA labeling index and iMVD were positively correlated with each other in prostatic carcinoma.

关 键 词：前列腺肿瘤 免疫组织化学 DNA倍体 PCNA iMVD 

分 类 号：R737.250.2[医药卫生—肿瘤]