石榴花多酚对糖尿病大鼠IL-6、TXB2及PPAR-γ mRNA基因表达的影响  

Experimental study of Punica granatum flower Polyphenol's effect on IL-6,TXB2's and PPAR-γ mRNA's expression in IR rats

作　　者：窦勤[1] 魏媛媛[2] 李郁[1] 闫冬[1] 阿迪力[1] 袁婷[1] 库热西.玉努斯 帕尔哈提.克热木 

机构地区：[1]新疆医科大学药理学教研室,新疆乌鲁木齐830001 [2]新疆医科大学生理学教研室,新疆乌鲁木齐830001 [3]新疆医科大学生化与分子生物学教研室,新疆乌鲁木齐830001

出　　处：《中国药理学通报》2010年第6期794-797,共4页Chinese Pharmacological Bulletin

基　　金：新疆维吾尔自治区科技攻关(含重大专项)和重点项目计划(No200733146)

摘　　要：目的观察石榴花多酚对小剂量链脲佐菌素加高脂饲料诱导的胰岛素抵抗模型大鼠IL-6、TXB2以及心肌组织PPAR-γ mRNA基因表达的影响。方法♂SD大鼠高脂饲料喂养1mon后注射小剂量STZ,2wk后测大鼠空腹血糖,血糖值>11.1者为造模成功的大鼠,将造模成功的大鼠随机分为模型组和药物干预组,另设空白对照组,药物干预1mon,常规测定各组大鼠给药前后的空腹血糖(FPG)和给药后各组大鼠的空腹血清胰岛素水平(Fins)、IL-6、TXB2,计算胰岛素敏感性指数(ISI)、胰岛素抵抗指数(Homa-IRI),提取心肌组织总RNA,采用逆转录PCR技术扩增PPAR-γ基因片段,检测其基因表达水平。结果石榴花多酚能降低模型大鼠FPG水平和Fins、IL-6、TXB2含量,增加PPAR-γmRNA基因表达,提高ISI,降低Homa-IRI。结论石榴花多酚对大鼠IR有一定程度的改善作用,其机制可能与提高IR大鼠心肌组织PPAR-γ基因的表达有关。Aim To investigate Punica granatum flower Polyphenol＇s effect on peroxisome proliferator-activated receptor-γ（PPAR-γ）mRNA＇s expression in heart and IL-6,TXB2＇s content of blood serum in insulin resistance （IR） rats which were induced by low dose of streptozotocin（STZ） and high calorie food.Methods Male SD rats were randomly divided into normal group,model group,greating group.The rats of normal group were fed with common food while the others with high calorie food and injected with low dose of STZ.Fasting plasma glucose（FPG）,fasting insulin（FIns）,IL-6 and TXB2 level of all rats were measured by routine methods,and insulin sensitivity index（ISI）was calculated accordingly.At the end of experimental treatment,the total mRNAs of heart tissues were extracted and the genic fragments of PPAR-γ were amplificated in order to detect their expression levels.Results Punica granatum flower Polyphenol could reduce the FPG,FIns,IL-6 and TXB2 level in model rats ,increase the PPAR-γ mRNA＇s expression and enhance ISI effectively.Conclusion Punica granatum flower Polyphenol can ameliorate IR conditon of the model rats and its mechanism is probably related to its effect of enhancing the genic expression level of PPAR-γ.

关 键 词：石榴花多酚 IR FINS IL-6 TXB2 PPAR-Γ 

分 类 号：R-332[医药卫生] R284.1