脂联素基因SNP+45 T/G单核苷酸多态性与冠心病的相关性研究  

Correlation of adiponectin gene SNP+45 T/G polymorphism with coronary heart disease

作　　者：徐丽[1] 凌文华[2] 

机构地区：[1]广东轻工职业技术学院食品系,广东广州510300 [2]中山大学北校区公共卫生学院营养系,广东广州510080

出　　处：《中国病理生理杂志》2010年第6期1064-1068,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金团队项目(No.015042)

摘　　要：目的:研究中国华南地区汉族人脂联素基因SNP+45T/G单核苷酸多态性与冠心病的相关性。方法:以153例冠脉造影证实为冠心病的非糖尿病患者为冠心病组,以73例健康者为对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析脂联素基因SNP+45T/G单核苷酸多态性与冠心病的相关性,ELISA法测定血浆脂联素水平。结果:冠心病组T/G+G/G型频率、G等位基因频率均高于对照组(P<0.05)。Logistic回归分析发现脂联素基因SNP+45 T/G+G/G型与冠心病正相关(OR:2.132,95.0%C I:1.034-4.397,P<0.05),血浆脂联素水平与冠心病的发病呈负相关(OR:0.868,95.0%C I:0.785-0.959,P<0.05)。结论:脂联素基因SNP+45 T/G+G/G型可能是华南地区汉族人冠心病的易感基因型。AIM： To investigate the relationship between adiponectin gene SNP ＋ 45 polymorphism and coro- nary heart disease （CHD） in south China Han population. METHODS： The nondiabetic CHD patients diagnosed by the coronary angiography were selected as CHD subjects （ 153 cases）, and 73 healthy adults served as normal control subjects. The polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP） was ,performed to identify the distribution pattern of adiponectin gene SNP ＋ 45 in all subjects. The levels of plasma adiponectin were measured by ELISA. RESULTS ： The frequency of T/G ＋ G/G genotype and G allele in CHD patients were significantly higher than those in control subjects （ P 〈 0. 05）. Logistic regression analysis revealed that the adiponectin gene SNP ＋ 45 T/G ＋ G/G genotype had a strong positive association with CHD （ OR ： 2. 132, 95. 0% CI ： 1. 034 - 4. 397, P 〈 0. 05 ）. The plasma adiponectin was negatively associated with CHD （ OR： 0. 868, 95.0% CI： 0. 785 - 0. 959, P 〈 0. 05 ）. CONCLUSION： The T/G ＋ G/G genotype was a possible risk factor for CHD in southern China Han population.

关 键 词：脂联素 基因 单核苷酸多态性 冠状动脉疾病 

分 类 号：R363[医药卫生—病理学]