针刀和电针对膝骨关节炎大鼠痛阈和中枢单胺类神经递质的影响  

Effects of acupotome and electro-acupuncture on pain threshold and monoamine-neurotransmitters of central nerves in rats with knee osteoarthritis

作　　者：嵇波[1] 郭长青[1] 金燕[1] 钟鼎文[1] 陈幼楠[1] 郭孟玮[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《中国病理生理杂志》2010年第6期1091-1095,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展计划(973)资助项目(No.2006CB504508);国家中医药管理局针灸特色疗法评价重点研究室资助项目

摘　　要：目的:对比观察针刀松解法、电针对膝骨关节炎(KOA)大鼠痛阈和中枢神经递质的影响。方法:80只SD大鼠被随机分为空白对照组、模型组、针刀组和电针组,4%木瓜蛋白酶溶液与0.3 mol/L半胱氨酸溶液(1∶1混置0.5 h)的混合液20μL,分别于造模第1 d、4 d、7 d注射于大鼠左侧膝关节腔中,4周后,治疗组分别以针刀松解法或电针治疗,共3周。采用光辐射热法检测大鼠痛阈,膝关节HE染色,ELISA法分别测定大鼠中脑、下丘脑、延脑、海马、脊髓等不同部位5-HT、NE、DA含量。结果:膝骨关节炎大鼠造模后痛阈显著降低(P<0.01),而针刀松解法或电针治疗后,第1周、第2周、第3周痛阈值均明显高于模型组(P<0.05,P<0.01);针刀和电针疗法可以改善大鼠膝骨关节炎病理组织损伤,可调节大鼠中脑、下丘脑、海马、脊髓等部位5-HT的合成与代谢,调节脊髓、下丘脑和延脑NA合成和代谢,调节中脑DA合成与代谢。结论:针刀和电针疗法对膝骨关节炎大鼠痛阈、组织形态学和中枢单胺类神经递质的含量有一定的调节作用。提示2种疗法可通过调节中枢神经5-羟色胺和儿茶酚胺类神经递质的合成和代谢的失衡状态,以减轻关节软骨损伤,缓解膝骨关节炎发生发展时出现的疼痛。AIM： To observe the effect of acupotome and electro- acupuncture on pain threshold and neurotransmitters of central nervous system in rats with knee osteoarthritis. METHODS： Eighty SD rats were divided randomly into 4 groups： control group, model group, acupotome group and electro -acupuncture group. The caroid （4%） and cysteine （0. 3 mol/L） were mixed at 1：1 for 0. 5 h and 20μL mixture was administered by injection to the left knee joint cavity of the rats on the 1 d, 4 d and 7 d respectively before the models were created. Four weeks later the rats in acupotome group and electro - acupuncture group were subject to apply the therapy of acupotome or electro - acupuncture for successive 3 weeks. Photoradiation was adopted to detect the pain tolerance threshold in the animals. The knee joints were stained with HE method. The contents of 5 - HT, NE and DA were detected in different sites of the midbrain, hypothalamus, medulla oblongata, hippocampus and spinal cord with ELISA. RESULTS ： The pain tolerance threshold of the rats with knee osteoarthritis significantly decreased as compared to that of the animals in control group （P 〈0.01 ）. The pain tolerance threshold of the rats in acupotome group and electro - acupuncture group markedly increased after the first three week therapy as compared to that in model groups （P 〈 0. 05, P 〈0. 01 ）. The results showed that the therapy of acupotome or electro-acupuncture greatly alleviated the pathological tissue injury caused by knee osteoarthritis. The therapy of acupotome or electro - acupuncture also regulated the synthesis and metabolism of 5 - HT in the mid - brain, hypothalamus, hippoeampus and spinal cord, the synthesis and metabolism of NA in the spinal cord, hypothalamus, and medulla oblongata, and regulated the synthesis and metabolism of DA in the mid - brain. CONCLUSION ： The therapy of acupotome or electro - acupuncture can regulate the pain threshold, histomorphology and the content of monoamine - in rats with knee osteoarthfiti

关 键 词：骨关节炎 针刀松解法 电针 5-羟色胺 儿茶酚胺类 

分 类 号：R363[医药卫生—病理学]