TNF-α单克隆抗体治疗大鼠肝肺综合征的机制研究  

Therapeutic mechanisms of tumor necrosis factor-α monoclonal antibody on hepatopulmonary syndrome in rats

作　　者：刘丽[1] 赵芝[1] 刘楠[1] 吴雅莉[2] 付洁[3] 

机构地区：[1]河北医科大学第二医院消化内科,河北省消化病重点实验室,河北省消化病研究所,河北省石家庄050000 [2]河北医科大学第二医院呼吸科,河北省石家庄050000 [3]河北医科大学第二医院检验科,河北省石家庄050000

出　　处：《中国病理生理杂志》2010年第6期1138-1141,共4页Chinese Journal of Pathophysiology

基　　金：河北省医学科学研究重点资助课题(No.08080)

摘　　要：目的:探讨肿瘤坏死因子-α抗体(TNF-α-McAb)抑制肝肺综合征大鼠TNF-α作用后肺组织诱导型一氧化氮合酶(iNOS)的表达情况,从而探讨TNF-α单克隆抗体对肝肺综合症治疗作用的机制。方法:采用胆总管结扎(CBDL)致大鼠肝肺综合征模型,将60只雄性SD大鼠随机分成假手术组、模型组和TNF-α-McAb干预组。采用HE染色观察肺组织病理变化,采用RT-PCR方法检测iNOS在肺组织中mRNA表达水平,免疫组织化学方法检测iNOS在肺组织中分布及含量的动态变化,采用Western blotting方法检测iNOS在肺组织中蛋白表达情况。结果:肺组织病理显示干预组病理改变炎症反应较模型组轻,TNF-α-McAb干预组大鼠肺组织中iNOS mRNA的表达、蛋白含量明显低于模型组。结论:肿瘤坏死因子-α单克隆抗体通过抑制iNOS的表达对肝肺综合征起到治疗作用。AIM ： To investigate the expression of iNOS in the lung of HPS rats treated with tumor necrosis factor -α monoclonal antibody （ TNF -α - McAb） and to investigate the therapeutic mechanism of TNF -α - McAb on hepatopulmonary syndrome. METHODS： Male Sprague -Dawley rats, weighing （250 ±25）g, were randomized to sham operation group, common bile duct ligation （CBDL） group and CBDL ＋ TNF -α McAb treatment group. Histopathological changes of the lung tissue were evaluated by hematoxylin and eosin staining. The mRNA expression of iNOS in the lungs of hepatopulmonary rats was examined by RT - PCR, while the changes of iNOS in the protein level were evaluated by immunohistochemistry and Western blotting. RESULTS ： The inflammatory responses in the CBDL rats treated with TNF -α - McAb decreased than that in CBDL group. Compared to CBDL group, the distribution of iNOS protein and the mRNA expressions in the lung tissue in TNF -α - McAb group were inhibited. CONCLUSION： TNF - α- McAb inhibits the expression of iNOS and plays a role in the treatment of HPS.

关 键 词：肿瘤坏死因子-α单克隆抗体 肿瘤坏死因子 诱导型一氧化氮合酶 肝肺综合征 

分 类 号：R575.2[医药卫生—消化系统]