三七总皂甙对脂肪变性L02肝细胞TG含量及LXRα mRNA表达的影响  

Effects of panax notoginseng saponins on content of triglyceride and the mRNA expression of LXRα in steatotic hepatocyte L02

作　　者：程少冰[1] 杨钦河[1] 张玉佩[1] 杨环文[1] 谢芳[1] 薛川松[1] 欧健[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632

出　　处：《中国病理生理杂志》2010年第6期1151-1155,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30973694)

摘　　要：目的:观察三七总皂甙(PNS)对脂肪变性L02肝细胞内甘油三酯(TG)含量及肝X受体α(LXRα)mRNA表达的影响,探讨其对脂肪变性肝细胞的降脂作用及机制。方法:采用50%小牛血清诱导L02肝细胞48 h建立肝细胞脂肪变性模型,采用MTT法测定PNS作用于脂肪变性肝细胞的适宜浓度,随后将其分为5组,模型组、自然恢复组、PNS低剂量组(10 mg.L-1)、PNS高剂量组(50 mg.L-1),并设正常组,除模型组继续予含50%小牛血清的RPMI-1640培养基培养外,余组均改予含10%小牛血清培养。药物作用24 h后,油红O染色观察肝细胞内脂滴变化,全自动生化仪检测肝细胞内TG含量,运用RT-PCR法检测肝细胞内LXRα mRNA的表达。结果:与正常组比较,油红O染色示模型组肝细胞内橘红色脂滴明显增加,并出现脂滴融合现象,模型组TG含量明显升高(P<0.01)。PNS治疗24 h后,PNS各治疗组与自然恢复组比较,肝细胞内TG含量均明显减少(P<0.05),以低剂量组下降更为显著(P<0.01);油红O染色显示,PNS低剂量组肝细胞内脂滴数减少最为明显。与正常组比较,模型组肝细胞LXRαmRNA的表达明显上调(P<0.01);与自然恢复组比较,PNS各治疗组肝细胞LXRα mRNA的表达量均有下降,以低剂量组下降显著(P<0.05)。结论:PNS能显著降低脂肪变性肝细胞内TG含量,减轻肝细胞脂肪变性。LXRα mRNA的高表达与肝细胞脂肪蓄积密切相关,PNS可能是通过下调LXRαmRNA的表达来改善肝细胞的脂肪变性。AIM： To study the effects of panax notoginseng saponins （PNS） on the content of triglyceride （TG） and the mRNA expression of liver X receptor α（LXRα） in steatotic hepatocyte L02. METHODS ： The cells of hepatocyte L02 were cultured with 50% bovine calf serum for 48 h and a model of steatosis of hepatocytes was established. The appropriate concentrations of PNS on steatotic hepatocytes were detected by MTT assay. The cells were divided into 5 groups： normal control group, model group, spontaneous recovery group, low concentration of PNS group （10 mg/L） and high concentration of PNS group （50 mg/L）. Meanwhile, hepatoeytes in model group were continuously cultured by RPMI - 1640 medium containing 50% bovine calf serum while others were cultured by RPMI - 1640 medium containing 10% bovine calf serum. After 24 h, the lipid droplets in the cells stained with oil red 0 were observed under microscope, the intracellular TG levels were determined by automatic biochemical analyzer and the mRNA expression of LXRα in hepatocytes was examined by RT - PCR. RESULTS ： Compared to normal control group, Oil red O staining presented numerous orange - red or red lipid droplets in the cytoplasm of human L02 hepatocytes in model group. The content of TG in model group were significantly increased （P 〈 0. 01 ）. After treated with PNS for 24 h, the content of TG in PNS treatment groups was significant decreased than that in spontaneous recovery group （ P 〈 0.05 ）, especially in low concentration of PNS group （ P 〈 0.01 ）. The accumulation of lipid droplets in low concentration of PNS group was decreased significantly. Compared to normal control group, the mRNA expression of LXRα in model group was significantly upregulated （P 〈 0. 01 ）. Compared to spontaneous recovery group, the mRNA expression of LXRα in PNS treatment groups declined in different levels, especially in low concentration group （ P 〈 0. 05 ）. CONCLUSION： The deposition of lipid in hepatocytes might be related

关 键 词：三七总皂甙 甘油三酯类 肝X受体Α 脂肪变性 L02细胞 肝疾病 非酒精性脂肪性 

分 类 号：R363[医药卫生—病理学]