致敏小鼠骨髓间充质干细胞体外培养及多向分化功能的测定  

Biological characteristics and induced differentiation of bone marrow-derived mesenchymal stem cells from sensitized mice in vitro

作　　者：洪冬玲[1] 吴燕峰[2] 许吕宏[1] 方建培[1] 

机构地区：[1]中山大学附属第二医院儿科,广东广州510120 [2]中山大学附属第二医院生物治疗中心,广东广州510120

出　　处：《中国病理生理杂志》2010年第6期1203-1207,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30872383)

摘　　要：目的:评价异基因脾细胞输注致敏的小鼠骨髓源性间充质干细胞(MSCs)的体外培养生长能力及其多向分化功能。方法:应用贴壁培养法体外培养间充质干细胞,流式检测其表面标志以及检测其成骨、成脂和成肌多向分化状况;结果:致敏小鼠骨髓源性MSCs与非致敏小鼠骨髓源性MSCs比较,形态学无差异且均表达CD29+、CD105+、CD44+和Sca-1+;CD34-、CD11b-;同时在相应的诱导条件下具有向成骨、成脂、成肌多向分化的能力。结论:异基因脾细胞输注致敏的小鼠,其骨髓源性MSCs的形态学和功能与正常小鼠的MSCs比较评估未见异常。AIM： To evaluate the biological characteristics and differentiating potentials of bone marrow -derived mesenchymal stem cells （MSCs） from sensitized mice by allogeneic splenocyte transfusion in vitro. METHODS ： Adherent culture method was applied for culturing the bone：marrow - derived MSCs from sensitized mice. The cell morphology was examined and the surface marker profiles were analyzed by flow cytometry. The differentiating potentials of the MSCs into osteogenic, adipogenic and myogenic lineages were explored. The bone marrow - derived MSCs from the normal mice were collected and served as controls. RESULTS： Both the bone marrow -derived MSCs from sensitized and normal mice were exhibited a homogeneous distinctive morphology and were positive for the surface markers CD29, CD105, CIM4 and Sca - 1, negative in CD 34 and CD11 b. The abilities of both MSCs to differentiate into osteogenic, adipogenic and myogenic pathways in the same condition were also observed. CONCLUSION： There is no difference in the biological characteristics and induced differentiating potentials between the sensitized mouse bone marrow - derived MSCs by allogeneic splenocytes transfusion and the MSCs from normal mice.

关 键 词：致敏小鼠 间充质干细胞 细胞分化 

分 类 号：R363[医药卫生—病理学]