大鼠局灶性脑缺血再灌注后凋亡诱导因子的表达与神经元凋亡的关系  

Relationship between expression of apoptosis inducing factor and neuronal apoptosis in rats with focal cerebral ischemia

作　　者：王彦平[1] 杨金升[1] 石向群[1] 张明磊[1] 司江华[1] 曲传勇[1] 范磊[1] 

机构地区：[1]兰州军区兰州总医院神经内科,兰州730050

出　　处：《中华神经医学杂志》2010年第6期582-585,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨大鼠局灶性脑缺血再灌注后凋亡诱导因子（AIF）的表达变化及与细胞凋亡的关系.方法 将Wistar大鼠分为假手术组（6只）和模型组（30只）.模型组大鼠采用线栓法建立大脑中动脉闭塞模型,假手术组大鼠插线较模型组浅,不造成大脑中动脉闭塞.观察假手术组及模型组缺血再灌注后6 h、24 h、48 h、72 h和7 d缺血半暗带AIF的表达,同时利用TUNEL法观察对应区域细胞凋亡的动态变化规律.结果模型组脑缺血再灌注6 h在缺血半暗带区AIF阳性细胞显著增加,再灌注48 h达到高峰[（130.47±11.32）个];各时相点均可见细胞凋亡,凋亡细胞数以再灌注48 h最多f（118.53±11.67）个];各组问比较差异均有统计学意义（JP〈0.05）.结论 局灶性脑缺血再灌注可致AIF表达增加.并引起细胞凋亡.Objective To study the relationship between the expression of apoptosis inducing factor （AIF） and neuronal apoptosis in rats with focal ischemia reperfusion.Methods Wistar rats were divided into sham-operated （n=6） and model （n=30） groups.Middle cerebral artery occlusion （MCAO）models were established by thread ligation.The expression of AIF in the ischemic penumbra was observed in the sham-operated and model groups 6 h and 1,2,3 and 7 d after the reperfusion; the changes of neuronal apoptosis in corresponding region were observed by TUNEL staining at the same time.Results AIF-positive cells in the ischemic penumbra were significantly increased in the model group 6 h after the ischemic reperfusion,reaching its peak level at 48 h of reperfusion （130.47±11.32 cells）.Cell apoptosis occurred at each time point of ischemic reperfusion and the percentage of apoptosis cells at 48 h ofreperfusion （118.53±11.67 ceils） was the highest among all the time points.Significant differences between the 2 groups were found between each 2 time points （P〈0.05）.Conclusion Focal ischemia reperfusion can induce the increased expression of AIF positive cells and neuronal apoptosis.

关 键 词：脑缺血 凋亡诱导因子 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]