小鼠逆行性遗忘动物模型的建立  

Establishment of retrograde amnesia model in mice

作　　者：汤建文[1] 李蕊[3] 范郑丽[2] 万子兵[1] 赵红梅[1] 隋建峰[1] 

机构地区：[1]第三军医大学基础部教学实验中心,重庆400038 [2]第三军医大学生理系,重庆400038 [3]第三军医大学西南医院康复理疗科,重庆400038

出　　处：《中华神经医学杂志》2010年第6期598-601,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30771769）

摘　　要：目的 探讨用电击、缺氧、麻醉等处理方法 建立小鼠逆行性遗忘动物模型的可行性及优劣.方法 将72只昆明小鼠分为对照组及电休克、缺氧、丙泊酚、电休克＋缺氧、电休克＋丙泊酚5个处理组.先给予各组相同的避暗训练以建立避暗行为,随后分别给予各处理组120～180 V电击、密闭容器内缺氧、腹腔注射0.3 mL丙泊酚、120～180 V电击＋密闭容器内缺氧、120～180 V电击＋腹腔注射0.3 mL丙泊酚相应处理.次日开始用暗箱观察各组小鼠的步入潜伏期,以分析避暗行为的变化.结果对照组小鼠在避暗训练后24 h（第4天）的步入潜伏期为（111.7±17.2）S,缺氧组、电休克＋缺氧组、电休克＋丙泊汾组与对照组相比差异均有统计学意义（P〈0.05）;电休克组、缺氧组、电休克＋缺氧组、电休克＋丙泊酚组4组均有部分小鼠步入潜伏期明显缩短至30 s以内,其发生率分别为43.8%、45.4%、66.7%、60%,而丙泊酚组步入潜伏期无明显变化.第5天、第8天观察显示,步入潜伏期缩短的小鼠中个别出现恢复.结论 电休克、缺氧、电休克＋缺氧、电休克＋丙泊酚处理后的小鼠中部分可出现逆行性遗忘表现,以电休克＋缺氧组建模的成功率最高：已出现逆行性遗忘的小鼠中部分可在后期恢复;单纯丙泊酚不能引起逆行性遗忘.Objective To explore the feasibility and advantages of establishment of retrograde amnesia （RA） model in mice with such methods as electric shock,anoxia,and anesthesia.Methods Kunming mice were divided into control group and 5 treatment groups,including treatments with electric shock,anoxia,propofol,electric shock＋anoxia,electric shock＋propofol.At first,every group received the same dark avoidance training to establish the behavior of dark avoidance,then the 5 treatment groups were treated with the methods of 120-180 V electric shock,anoxia within a closed container,intraperitoneal injection of 0.3 mL of propofol,electric shock＋anoxia,electric shock＋propofol,respectively.Next day,step-in latency （Lat） of mousse were measured with the dark chamber in all groups and changes of dark avoidance behavior were analyzed.Results The Lat in the control group 24 h after dark avoidance training was （111.7＋17.2） s.In the treatment groups of electric shock,anoxia,electric shock＋anoxia,electric shock＋propofol,significantly shortened Lat,which limited to 30 s in some mice 24 hatter corresponding treatment,was observed as compared with that in the control group （P〈0.05）.Except for the propofol treatment group did not changed obviously,the incidence rate of shortened Lat was 43.8%,45.4%,66.7% and 60% in the electric shock treatment group,anoxia treatment group,electric shock＋anoxia treatment group,and electric shock＋propofol treatment group,respectively.On the 5th and 8th d,some mice recovered from the shortened Lat.Conclusion RA model can be established successfully in some mice treated with electric shock,anoxia,electric shock＋anoxia,electric shock＋propofol and the highest modeling rate was found in the electric shock＋anoxia treatment group.RA can recover in the later stage in some modeling mice and use of pmplfol alone call not induce RA.

关 键 词：逆行性遗忘 电休克 缺氧 丙泊酚 动物模型 

分 类 号：R-332[医药卫生]