褪黑素前后干预对左旋精氨酸诱导的实验性急性坏死性胰腺炎大鼠肺胰组织损伤的保护作用  

The protective effect of melatonin pre-and post-treatment on experimental acute necrotizing pancreatitis with L-arginine-induced in rats

作　　者：牛桂军[1,2] 唐国都[1] 梁志海[1] 钟卫一[1] 黄文福[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,广西南宁530021 [2]广西壮族自治区南溪山医院消化内科

出　　处：《胃肠病学和肝病学杂志》2010年第6期557-560,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：广西自然科学基金资助项目(No.桂科自0728107)

摘　　要：目的探讨褪黑素前、后干预对急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肺和胰腺组织的保护作用。方法将96只SD大鼠随机分成对照组(C组,n=24)、急性坏死性胰腺炎组(A组,n=24)、褪黑素前干预组(M1组,n=24)和后干预组(M2组,n=24)。A组用6%的左旋精氨酸(L-Arginine,L-Arg)腹腔内注射,每次25mL/kg体质量,共3次,注射间隔1h,诱导ANP模型,在首次注射L-Arg前0.5h腹腔注射生理盐水20mL/kg体质量1次;C组同法注射相当于A组各次注射用量的等容积生理盐水;M1和M2组分别在诱导胰腺炎前和后0.5h腹腔内注射0.25%褪黑素(20mL/kg体质量)干预。在末次注射L-Arg后的第6h、第12h和第24h3个时点分批处死大鼠,观察各组对应各时点胰腺和肺组织病理学改变的变化。结果 A组大鼠胰腺和肺组织病理损伤在各时点较C组明显加重(P<0.01);M1组胰腺和肺组织病理损伤较A组减轻,尤以第6h、第12h显著(P<0.01或0.05)。M2组上述改变介于A组和M1组之间。M1组较M2组病变轻;在第24h,A组血清淀粉酶较C组显著升高(P<0.01);M2组淀粉酶较A组降低(P<0.05);M1较A组低。结论在ANP肺组织损伤程度与胰腺组织损伤程度密切相关。褪黑素在一定程度上减轻ANP的胰腺和肺损伤。M1组对肺和胰腺组织的保护作用明显强于M2组。Objective To assess the protective effect of melatonin pre-and post-treatment on experimental acute necrotizing pancreatitis（ANP）with L-arginine（L-Arg）-induced in rats.Methods Ninety-six Spraque-Dawley male rats were randomly divided into four groups C,A,M1 and M2.ANP were induced by intraperitoneally injecting three times with 6% L-Arg at a dose of 25 mL/kg body weight at an interval of 1 h in group A.Group C received normal saline alone with the same methods.Group M1 and M2 were treated by injecting intraperitoneally with a dose of 20 mL/kg body weight 0.25% melatonin at 30 min before and after L-Arg induced.Rats were killed at the 6th,12th and 24th hour after the last time of L-Arg injection.The pathological changes of pancreatic and lung tissues were respectively analyzed and scored according to Kusser＇s and Lei weizhang＇s standard.Results With time going,the pathological injuries of the pancreas and lung in group A were significantly more severe than those in group C（P0.01）.However,injuries in group M1 were significantly more mild than those in group A,especially within the 6th and 12th（P0.01 or 0.05）.The degree of pathological injury in M2 was between the degree of group A and M1,and the changes in group M1 were more mild than those in group M2.At the 24th hour,the serum amylase in group A was significantly higher than that in group C（P0.01）,but M1 group was lower than that in group A,the serum amylase in group M2 was significantly lower than that in group A（P0.05）.Conclusion Lung injury is closely related to pancreatic tissue injury in ANP.Melatonin may reduce the lung and pancreatic injury in ANP.The protective effect of pancreatic and lung in group M1 was stronger than group M2.

关 键 词：急性坏死性胰腺炎 急性肺损伤 褪黑素 

分 类 号：R576[医药卫生—消化系统]