类似人弥漫型大B细胞淋巴瘤小鼠模型的免疫学特征  

Immuno-characterization of Mouse Model Similar to Human Diffuse Large B cell Lymphoma

作　　者：刘芳[1,2] 张弓 周新华 刘靖[1] 岳赟[1] 赵彤 

机构地区：[1]佛山科学技术学院医学院基础医学部,广东佛山528000 [2]广东省分子肿瘤病理学重点实验室,广东广州510515

出　　处：《中国实验血液学杂志》2010年第3期655-659,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(编号30770908);广东省自然科学基金博士启动项目(编号9452800001002250)资助

摘　　要：本研究建立类似人弥漫型大B细胞淋巴瘤的BALB/c小鼠模型并探索其免疫学特征。将鼠源性B淋巴瘤细胞株(A20细胞)接种于同源BALB/c小鼠以建立B细胞淋巴瘤鼠模型。实验分为3组:成瘤小鼠组,未成瘤小鼠组和正常小鼠组(对照组)。用流式细胞术检测肿瘤细胞CD抗原表达及成瘤小鼠、未成瘤小鼠和对照正常小鼠的外周血和脾脏的T/B淋巴细胞亚群比例。结果表明:在成功构建病理学形态类似人弥漫大B细胞淋巴瘤的BALB/c鼠模型肿瘤组织中,检测到CD3、CD4、CD8、CD19、CD30阳性细胞的比例分别为(49.27±23.75)%,(6.07±3.65)%,(51.2±23.1)%,(67.06±16.39)%,(37.93±17.03)%,与接种前A20细胞相比,其CD3和CD8阳性细胞比例显著升高,CD19阳性表达比例显著下降(p<0.05)。成瘤小鼠外周血淋巴细胞亚群阳性表达比例较正常小鼠有显著差异,其CD3和CD4阳性细胞比例显著降低(p<0.05)。未成瘤小鼠脾脏淋巴细胞亚群的阳性表达比例与正常小鼠相比,CD3、CD4、CD8阳性细胞比例降低,而CD19阳性细胞比例升高(p<0.05)。结论:本研究为在有免疫功能的小鼠体内进行B细胞淋巴瘤相关研究提供了免疫相关实验依据。This study was purposed to establish the BALB/c mouse model with similar diffuse large B cell lymploma and to investigate the immuno-characteritics of this model. The experiments were divided into 3 groups including group 1 （ BALB/c mice with tumor resulting from injection of A20 cells ）, group 2 （ BALB/c mice without tumor formation resulting from injection of A20 cells ） and group 3 （ normal BALB/c mice ）. The CD antigen expression of tumor cells in vitro and in vivo, and the ratios of T/B lymphocytes in peripheral blood and spleen tissue of 3 groups were detected by flow cytometry. The results showed that the animal models were successfully established, the pathologic characteristic of tumor cells from animal model were similar to human diffuse large B cell lymphoma. The positive expression levels of CD3, CD4, CD8, CD19 and CD30 in tumor tissue were （49.27 ± 23.75 ） %, （6.07 ± 3.65 ） %, （51.2 ±23.1 ） %, （ 67.06 ± 16.39 ） % and （ 37.93 ± 17.03 ） % respectively; as compared with A20 cells, the expression levels of CD3 and CD8 significantly increased, while the expression level of CD19 significantly decreased （p 〈 0.05 ）. The obvious decrease of CD3 and CD4 expression was observed in peripheral blood of mice with tumor as compared with normal mice （p 〈 0.05 ）. The expression levels of CD3, CD4 and CD8 decreased while the expression level of CD19 increased in the spleen cells of mice without tumor formation as compared with normal mice （ p 〈 0.05 ）. It is concluded that the immunophenotypes of A20 ceils and successfully established animal models can be useful studying human B cell lymphoma.

关 键 词：弥漫大B细胞淋巴瘤 A20细胞株 BALB/C鼠 免疫表型 

分 类 号：R733[医药卫生—肿瘤]