青蒿素对人鼻咽癌CNE细胞的放射增敏作用及机制的研究  

Radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells

作　　者：唐艳[1] 曹建平[1] 樊赛军[1] 朱巍[1] 张旭光[2] 陈遐林[1] 纪蓉[1] 潘春燕[1] 周媛媛[1] 封阳[1] 江庆[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室,215123 [2]徐州市肿瘤医院

出　　处：《中华放射医学与防护杂志》2010年第3期280-282,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（30670632）;长江学者和创新团队发展计划资助（IRT0849）

摘　　要：目的 探讨青蒿素对人鼻咽癌CNE细胞的放射增敏作用.方法 应用甲基偶氮唑盐（MTT）比色法检测青蒿素对鼻咽癌CNE细胞的抑制效应;克隆形成实验观察青蒿素对CNE细胞的放射敏感性的影响;流式细胞术测定照射后CNE细胞周期的再分布.结果 青蒿素对CNE细胞的抑制率呈药物剂量依赖性增加.青蒿素能明显增加.γ射线对CNE细胞的克隆形成抑制作用,1 μmol/L青蒿素对CNE细胞的放射增敏比（SER）为1.26.青蒿素能够去除γ射线照射后CNE细胞的G2/M期阻滞,与单纯6 Gy照射组相比,青蒿素＋照射组G：/M期阻滞减少.结论 青蒿素通过减弱辐射诱导的G2/M期阻滞,从而增加γ射线对CNE细胞的杀伤作用.Objective To investigate the radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells in vitro.Methods CNE human nasopharyngeal carcinoma cell line was used in this study.Cell growth kinetics was determined by MTT assay.Effect of the drug on radiosensitivity of CNE cells was analyzed by clonogenic assay.The change of cell cycle was measured by flow cytometry.Results The inhibition of CNE cells growth by artemisinin was increased with concentrations.Artemisinin （1 μmol/L）could enhance the radiosensitizing effects on CNE cell line,and the sensitizing enhancement ratio（SER）was 1.26.Artemisinin abrogated radiation-induced G2/M arrest of the tested CNE cells.Compared with the radiation alone group,the proportion of G2/M phase cells increased in radiation combined with drug group.Conclusions Artemisinin could reduce radiation-induced G2/M arrest and enhance the cytotoxicity of γ-irradiation on the CNE ceils.

关 键 词：青蒿素 鼻咽癌 放射增敏 细胞周期 

分 类 号：R739.63[医药卫生—肿瘤]