外源精脒和精胺可诱导大鼠早期再生肝抗酶的表达  

Eexogenous spermidine and spermine induce the expression of antizyme in early regenerating rat liver

作　　者：李恩中[1] 陈燕芳 索世英[3] 宁黔冀[3] 

机构地区：[1]黄淮学院农林科学院,河南驻马店463000 [2]郑州澍青医学高等专科学校临床医学系,郑州450064 [3]河南师范大学生命科学学院生理室,河南新乡453002

出　　处：《解剖学报》2010年第3期387-390,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30340042);河南省自然科学基金资助项目(092300410200;102300410036);黄淮学院青年骨干教师资助计划

摘　　要：目的探讨精脒和精胺对大鼠早期再生肝抗酶(AZ)表达的影响,及在肝再生中的作用。方法外源多胺(溶于0.9%NaCl)皮下注射雄性SD大鼠(180～200g),进行部分肝切除(PH)诱导。采用RT-PCR和Western blotting方法进行PH后大鼠再生肝中AZ基因转录量和蛋白表达量的分析。结果对照组完整肝脏(PH后0h)中,AZ基因转录量、蛋白表达量较低,PH后均快速升高,3h达到峰值,5h出现明显下降,7h再次升高并达到峰值,之后缓慢下降。外源多胺处理后,两种剂量精脒(0.03mg/kg和0.15mg/kg)和精胺(0.06mg/kg和6mg/kg)处理组AZ mRNA及蛋白表达水平变化趋势与对照组相似,但低剂量处理组远远低于相应时间点高剂量处理组。精胺的作用效果更明显,作用时间更持久。结论外源多胺对大鼠早期再生肝AZ mRNA及蛋白表达具有剂量依赖性促进作用,而精胺的作用较强,精脒较弱。Objective To study the roles of spermidine and spermine in affecting antizyme expression in early regenerating rat liver. Methods Adult male SD rats were surgically rendered partial hepatectomy via a modification of a procedure reported by Higgins and colleagues （1931）, then they were treated with different dosages of exogenous polyamines （dissolved in 0.9% NaCl） s.c. Antizyme （AZ） gene transcription and protein expression in regenerating liver were evaluated by using the RT-PCR and Western blotting assay respectively. Results In intact liver （0 hour after partial hepatectomy） of the control group, the levels of AZ mRNA transcription and protein expression were very low. In regenerating liver induced by partial hepatectomy, AZ mRNA content and protein expression increased quickly, peaked at 3 hours, followed by significant decreases at 5 hours, afterwards, it reached another peak at 7 hours, and then decreased gradually. In rats administered with different doses of spermidine and spermine, the change tendency of AZ mRNA level and protein expression in regenerating liver paralleled with those of control group. But the levels in low-dose-treated-group were greatly lower than that in high-dose-treated-group at each time point throughout the whole experiment. The actions of spermine were more obvious and longer. Conclusion Exogenous polyamines can induce antizyme express dose-dependently in early regenerating of rat liver, and the effects of spermine were more powerful than that of spermidine.

关 键 词：精脒 精胺 抗酶 再生肝 反转录-聚合酶连法 免疫印迹法 大鼠 

分 类 号：Q71[生物学—分子生物学]