益气补肾颗粒治疗对微小残留白血病患者CD4^+CD8^(high) T淋巴细胞和自然杀伤细胞的影响  

Effect of Yiqi Bushen Granule on the Peripheral CD4^+CD8^(high) T-lymphocyte and Natural Killer Cell in Patients with Minimal Residual Leukemia

作　　者：许勇钢[1] 麻柔[1] 杨晓红[1] 唐旭东[1] 孙淑贞[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中国中西医结合杂志》2010年第6期571-574,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30472284);首都医学发展科研基金(中医药)重大课题资助项目(No.I-1)

摘　　要：目的探讨益气补肾颗粒治疗后CD4+CD8highT淋巴细胞的变化及其对微小残留白血病(mini-mal residual leukemia,MRL)预后的意义。方法用多参数流式细胞技术比较分析36例MRL患者应用益气补肾颗粒治疗前后CD4+CD8 highT淋巴细胞亚群和自然杀伤细胞(NK细胞)改变。结果 (1)MRL患者3年持续完全缓解率、生存率分别为75.0%、80.5%,5年持续完全缓解率、生存率分别为63.9%、72.2%。(2)与治疗前比较,治疗后的MRL的CD4+CD8highT淋巴细胞百分率(%)和绝对值显著上升(P<0.01);(3)与治疗前比较,治疗后MRL的NK细胞百分率(%)和绝对值均显著上升(P<0.05,P<0.01)。结论益气补肾颗粒可能通过调节机体自身免疫功能,提高CD4+CD8highT和NK细胞的绝对数量,杀伤或抑制体内残存白血病细胞,延长MRL患者的无病生存期。Objective To explore the effect of Yiqi Bushen Granule （YBG） on CD4＋CD8high T lymphocyte in patients with minimal residual leukemia （MRL） and to probe its prognosis after treatment. Methods The changes of CD4＋CD8highT lymphocyte subgroup and natural killer （NK） cells in 36 patients （16 males and 20 females） received long-term （3 months） YBG therapy before and after treatment were analyzed using multi-parameter flow cytometry and controlled by those in 34 healthy persons （19 males and 15 females）. Results The 3-year and 5-year complete remission rates in MRL patients were 75.0% and 63.9%; their 3-year and 5-year survival rates were 80.5% and 72.2%,respectively; as compared with the baseline,the absolute value and percentage of CD4＋CD8high T lymphocyte and NK cells were all significantly raised after treatment （P0.05 or P0.01）. Conclusion YBG could prolong the disease-free survival in MRL patients by way of regulating the immune function of organism,elevating the amount of CD4＋CD8high T lymphocyte and NK cells to kill or suppress the residual leukemic cell in body.

关 键 词：微小残留白血病 益气补肾颗粒 CD4+CD8highT淋巴细胞 自然杀伤细胞 

分 类 号：R733.7[医药卫生—肿瘤]