类固醇激素依赖型溃疡性结肠炎缓解期IL-23表达的研究  

Expression of IL-23 in remission of steroid-dependent ulcerative colitis

作　　者：邱冰峰[1] 王志勇[1] 申定珠[2] 

机构地区：[1]杭州师范大学附属医院消化科,310015 [2]上海中医药大学附属曙光医院

出　　处：《中华消化内镜杂志》2010年第6期303-306,共4页Chinese Journal of Digestive Endoscopy

基　　金：浙江省医药卫生科学技术基金（No：2007B176）：杭州卫生科技计划项目（No：2008B0032）;浙江省教育厅科研计划项目（Y200908063）

摘　　要：目的 探讨IL-23在缓解期类固醇激素依赖型溃疡性结肠炎患者结肠组织中表达的病理意义.方法 采用Western blot分析及免疫组化SABC法检测15例缓解期激素依赖型溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达情况,予统计学软件统计分析,并以30例缓解期的一般溃疡性结肠炎患者（15例SASP维持治疗,15例强的松维持治疗）炎症修复区结肠组织及10例正常结肠黏膜组织为对照组.结果 与正常对照组比较,SASP维持治疗及强的松维持治疗缓解期一般溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达均轻度升高（P〉0.05）,而缓解期激素依赖型溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达显著高于一般溃疡性结肠炎组（P〈0.01）.结论 IL-23的过度表达可能在溃疡性结肠炎类固醇激素依赖发病机制中起关键作用.Objective To investigate the pathological significance of expression of IL-23 in colon tissues of patients with steroid-dependent uncreative colitis at remission.Methods Expression of IL-23 was measured by means of Western Blot and immunohistochemistry SABC in inflammation repairing areas from 15 patients with steroid-dependent ulcerative colitis at remission, 30 patients with common ulcerative colitis at remission （15 treated with SASP and 15 with prednisone） and 10 normal colon tissues.The results were analyzed by SPSS 16.0.Results Compared with normal control, expression of IL-23 in patients of SASP maintenance therapy and prednisone with common ulcerative colitis were slightly increased （ P 〉 0.05）, which was significantly lower that of steroid-dependent specimens （ P 〈 0.01）.Conclusion Over-expression of IL-23 may plas a key role in the pathogenesis of steroid-dependent ulcerative colitis.

关 键 词：结肠炎 溃疡性 类固醇 白细胞介素23 

分 类 号：R574.6[医药卫生—消化系统]