托吡酯对急性脑缺血后大鼠脑皮层氨基酸类神经递质含量的影响及神经保护作用  

Influence of topiramate on amino acids neurotransmitter of cerebral cortex in rats with cerebral ischemia reperfusion injury and neuroprotective effect

作　　者：刘姜冰[1] 石静萍[2] 赵薛旭[2] 李作汉[2] 

机构地区：[1]扬州市第一人民医院神经内科,扬州225001 [2]南京脑科医院神经内科,南京210029

出　　处：《重庆医科大学学报》2010年第5期692-694,共3页Journal of Chongqing Medical University

摘　　要：目的:探讨托吡酯(Topiramate,TPM)对脑缺血后大鼠脑组织内γ-氨基丁酸(γ-aminobutyric,GABA)、氨酸(Glutamin acid,glu)含量及神经功能变化的影响。方法:采用线栓法建立大鼠大脑中动脉闭塞(Middle cerebral artery occlusion,MCAO)模型,TPM组动物分别于插线和再灌注时腹腔注射新鲜配置成的TPM混悬液(8mg/ml,80mg/kg)。进行神经功能评分,TTC染色测梗死体积百分比,高效液相色谱分析仪测脑组织内GABA、glu的含量。结果:TPM组的梗死体积较单纯缺血再灌注组减少41.8%,;TPM组神经功能评分与缺血再灌注组比有显著改善;TPM组脑组织中GABA含量与单纯缺血再灌注组相比明显升高(P<0.05),glu明显降低(P<0.05)。结论:托吡酯有一定的神经保护作用,其可能机制为干扰了脑缺血再灌注损伤中兴奋性氨基酸glu的代谢,减少了神经细胞兴奋性毒性损伤。提高了脑内GABA的含量,进一步平衡glu的兴奋性作用。Objective：To investigate the neuro protective effects of topiramate on the changes of the nerve function deficiency scores and the influence of it on the content of amino acids neurotransmitter of cerebral cortex of rats with cerebral ischemia.Methods：Male SD rats were subjected to right middle cerebral artery occlusion for 2h and reperfusied for 24 h.Rats in the treatment group were injected with topiramate suspl（8mg/ml,80mg/kg）at the begining of the artery occlusion and the reperfusion.The nerve function deficiency scores were measured.The infarct volume was evaluated through TTC staining.The content of amino acids of cerebral cortex was estimated by the method of high performance liquid chromatography（HPLC）with fluorescent detection.Results：The infarct volume was significantly reduced by 41.8% in the TPMtreated group compared with that in the control group.There were significant differences in the neurological impairment scores between the treatment group and the ischemia group.TPM significantly decreased the content of Glu（P〈0.05）and increased the content of GABA（P〈0.05）.Conclusion：Topiramate maybe have some neuroprotective effect,which is probably related with its regulation on the relative equilibrium of Glu and GABA.

关 键 词：大鼠大脑中动脉闭塞模型 γ-氨基丁酸 谷氨酸 

分 类 号：R743.33[医药卫生—神经病学与精神病学]