缺血/再灌注大鼠脑和血浆中α-突触核蛋白含量的变化  

Change of α-Synuclein Level in Rat Brain and Plasma after Ischemia/Reperfusion

作　　者：贾春松[1] 李昕[1] 李尧华[1] 刘光伟[1] 殷娟娟[1] 吴燕川[2] 于顺[1] 

机构地区：[1]首都医科大学宣武医院老年病研究所神经生物学研究室教育部神经变性病重点实验室 [2]首都医科大学宣武医院中心实验室

出　　处：《首都医科大学学报》2010年第3期368-372,共5页Journal of Capital Medical University

基　　金：国家高技术研究发展计划(863计划)重大项目(2006AA02A408);北京市自然科学基金重大项目(7050001);北京市属高等学校人才强教计划资助项目(PHR200907113)~~

摘　　要：目的探讨缺血/再灌注大鼠脑和血浆中α-突触核蛋白(α-Synuclein,α-Syn)含量的变化。方法将30只雄性Wistar大鼠用随机数字表法分成假手术对照组(10只)和缺血/再灌注组(20只)。用线栓法制作大脑中动脉梗阻(middle cerebral artery occlusion,MCAO)模型,缺血1.5h后,再恢复灌注24h;用Western blotting法和ELISA法检测大脑缺血半暗带、梗死核心区和血浆中α-Syn的含量变化。结果缺血半暗带α-Syn表达量显著增加,而梗死核心区和血浆中α-Syn无显著性变化。结论缺血/再灌注可增加缺血半暗带中α-Syn的含量。Objective To explore the change of α-synuclein（α-Syn） in rat brain and plasma after ischemia/reperfusion. Methods Thirty adult male Wistar rats were randomly divided into 2 groups：sham operation group（10 cases） and ischemia/reperfusion group（20 cases）. A model of middle cerebral artery occlusion（MCAO） was induced by the intraluminal suture for the latter group. After 1.5 h ischemia,reperfused for 24 h,then the level of α-Syn in the plasma and penumbra,infarct core and their controls were measured by Western blotting and ELISA method. Results The level of α-Syn was increased significantly in the penumbra,but did not change obviously in the infarct core or plasma. Conclusion α-Syn was increased in the penumbra of brain after ischemia/reperfusion.

关 键 词：Α-突触核蛋白 缺血/再灌注 缺血半暗带 梗死核心区 血浆 

分 类 号：R743[医药卫生—神经病学与精神病学]