中国牛病毒性腹泻病毒JZ05-1分离株全基因测序及分析  

Genome Sequencing and Analysis of the Bovine Viral Diarrhea Virus-2 Strain JZ05-1 Isolated in China

作　　者：李庆超[1] 苗利光[1] 李海涛[1] 刘艳环[1] 张广雷[2] 肖佳美[1] 

机构地区：[1]中国农业科学院吉林特产研究所,吉林市132109 [2]江苏科技大学,镇江212018

出　　处：《病毒学报》2010年第3期238-243,共6页Chinese Journal of Virology

基　　金：国家科技支撑计划项目;(2006BAD06B06)

摘　　要：牛病毒性腹泻病毒为瘟病毒属成员,呈世界性分布并在乳/肉牛业中造成严重经济损失。本研究利用MD-BK细胞增殖一株分离于我国吉林地区的牛源牛病毒性腹泻病毒JZ05-1毒株,观察发现其具有典型的致细胞病变效应。使用逆转录-聚合酶链式反应(RT-PCR)方法分别扩增覆盖基因组全长的八个片段并测序,拼接获得该病毒的全基因组序列长12285核苷酸(nt),GenBank登录号:GQ888686。该病毒基因组具有编码多聚蛋白的一个11694nt可读框,5'非翻译区(UTR)长387nt,3'非翻译区长204nt。分别分析BVDVJZ05-1的5'-UTR序列和全基因组序列,发现该病毒属于BVDV-2a亚型。BVDV-2型多数毒株之间的相似性约为96%,与JZ05-1全基因组序列相似性最高的加拿大p11Q毒株和中国XJ-04毒株的相似性为90%和91%。因此,JZ05-1全基因组序列与BVDV-2型其他毒株的全基因组序列具有较大差异。Bovine viral diarrhea virus （BVDV） is a member of the genus Pestivirus,which is a widespread problem for beef and dairy herds,and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin,China generated cytopathic effect （CPE） in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced,the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results,the JZ05-1 genome was composed of 12 285 nucleotides in length（GenBank accession No. GQ888686）,which could be divided into three regions：a 387 nt 5＇-untranslated region （UTR）,a 11694 nt single large open reading frame encoding a polyprotein,and a 204 nt 3＇-UTR. The 5＇-UTR and genome sequences were analyzed by sequence alignment and construction of phylogenetic trees. The strain JZ05-1 was classified as BVDV type 2a. The BVDV-2 strain JZ05-1 genome showed high similarity to the p11Q isolated in Canada and the XJ-04 isolated in China,with 90% and 91% identity in nucleotide sequence,respectively. Compared with the similarity within the BVDV-2 genotype （96%）,the JZ05-1 had low sequence similarity to other BVDV-2 strains.

关 键 词：BVDV 全基因组 基因型 相似性 

分 类 号：S852.65[农业科学—基础兽医学]