溶血磷脂酸对人卵巢癌细胞P120catenin表达的影响  

Effect of Lysophosphatidic Acid on Expression of P120catenin in Human Ovarian Cancer Cells

作　　者：姜歆[1] 彭真年[1] 熊学华[1] 孙迪[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016

出　　处：《中国生物制品学杂志》2010年第6期605-608,612,共5页Chinese Journal of Biologicals

摘　　要：目的观察溶血磷脂酸(Lysophosphatidic acid,LPA)对人卵巢癌细胞SKOV3 P120catenin(P120ctn)表达的影响,探讨LPA和P120ctn在卵巢癌发生发展中的作用机制。方法将不同浓度的LPA作用于SKOV3细胞,MTT法检测细胞的增殖率;以最佳浓度的LPA及不同浓度的酪氨酸蛋白激酶抑制剂Genistein作用于SKOV3细胞,MTT法检测细胞的增殖抑制率;将SKOV3细胞分为3组:对照组、LPA组和LPA+Genistein组,分别于培养6、12、24h,采用免疫荧光细胞化学法和Western blot分析P120ctn表达的变化,RT-PCR法检测P120ctn基因mRNA的转录水平。结果 20μmol/LLPA作用24h促进SKOV3细胞增殖的作用最强,而200μmol/LGenistein对LPA促进SKOV3细胞增殖有明显的抑制作用。LPA可诱导SKOV3细胞膜中P120ctn的表达减少,胞质中的表达增多;而Genistein与LPA共同作用后,P120ctn在细胞膜中的表达增多,而在胞质中的表达减少。结论 LPA可诱导SKOV3细胞中P120ctn表达的改变,其机制可能与相关的酪氨酸蛋白激酶磷酸化有关。Objective To observe the effect of lysophosphatidic acid（LPA）on expression of P120catenin（P120ctn）in human ovarian cancer SKOV3 cells and investigate the action mechanism of LPA and P120ctn in onset and development of ovarian cancer.Methods SKOV3 cells were treated with LPA at various concentrations,and determined for proliferation rate by MTT method.SKOV3 cells were treated with LPA at optimal concentration plus tyrosine protein kinase inhibitor Genistein at various concentrations respectively,and determined for proliferation-inhibiting rate by MTT method.SKOV3 cells were divided into control,LPA and LPA ＋ Genistein groups,in which the expression of P120ctn were analyzed by immunofluorescent cytochemical method and Western blot,and transcription of P120ctn mRNA by RT-PCR 6,12 and 24 h after culture.Results The proliferation rate of SKOV3 cells reached a peak value 24 h after treatment with 20 μmol /L LPA.However,200 μmol /L Genistein significantly inhibited the promoting effect of LPA on SKOV3 cell proliferation.The expression level of P120ctn in membrane of SKOV3 cells treated with LPA decreased,while that in cytoplasm increased.However,the expression level of P120ctn in membrane of SKOV3 cells treated with LPA ＋ Genistein increased,while that in cytoplasm decreased.Conclusion LPA may induce the change of P120ctn expression in SKOV3 cells by a potential mechanism associated with the phosphorylation of the relevant tyrosine protein kinase.

关 键 词：溶血磷脂素类 P120catenin 蛋白酪氨酸激酶 蛋白激酶抑制剂 卵巢肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]