PEP-1-SOD1预处理对脑梗死后小鼠海马神经元的保护  

Neuropretection of PEP-1-SOD1 fusion protein against cerebral infarction in murine hippocampal region

作　　者：董敏[1,2] 席刚明[1] 张正洪[1] 

机构地区：[1]郧阳医学院附属人民医院神经内科,湖北十堰442000 [2]湖北省襄樊市中医院麻醉科

出　　处：《重庆医学》2010年第12期1487-1488,1491,I0001,共4页Chongqing medicine

基　　金：湖北省高等学校科学研究青年发展项目(98B042)

摘　　要：目的研究PEP-1-铜,锌超氧化物歧化酶(PEP-1-SOD1)预处理对脑梗死后小鼠海马神经元的保护作用。方法健康雄性成年昆明小鼠随机分为假手术组、模型组以及PEP-1-SOD1预处理组。线栓法制作永久性大脑中动脉闭塞(PMCAO)模型。假手术组以及模型组在手术前30min腹腔注射生理盐水200μL,PEP-1-SOD1预处理组手术前30min注射PEP-1-SOD1200μg。手术后24h分离梗死侧海马组织,TUNEL染色测定细胞凋亡,黄嘌呤氧化酶法测定SOD1活性,TBA法测定丙二醛(MDA)含量。结果手术后24h与模型组相比,PEP-1-SOD1预处理组凋亡细胞数量明显减少(P<0.01),SOD1活性升高,MDA含量下降(P<0.05)。结论 PEP-1-SOD1预处理可以有效减轻小鼠脑梗死后海马神经元损伤。Objective To explore the neuropretection of PEP-1-SOD1 against the cerebral infarction in murine hippocampal region.Methods The mice were assigned randomly into sham-operated group,model group and PEP-1-SOD1 pre-treatment group,the model of permanent middle cerebral artery occlusion（PMCAO）was set up.The mice were intraperitoneally injected 200 μL saline in sham-operated group and model group or 200 μg PEP-1-SOD1 in PEP-1-SOD1 pre-treatment group 30 min before operation,respectively.After 24 h of surgery,the animals were sacrificed and the hippocampal tissues were dissected for TUNEL stained,SOD1 activity and MDA amount.Results After 24 h of surgery,compared with model group,the apoptotic cells in PEP-1-SOD1 pre-treatment group were ameliorated（P0.01）,the activity of SOD1 was increased and the amount of MDA was decreased（P0.05）.Conclusion Pretreatment with PEP-1-SOD1 fusion protein can attenuate the neurons damage resulted from the cerebral infarction.

关 键 词：PEP-1-SOD1 脑梗死 保护作用 

分 类 号：R743[医药卫生—神经病学与精神病学]