绿粘帚霉F051产几丁质酶条件优化及其对三种病原菌的作用  

The Optimization of Fermentation Conditions of Chitinase-producing Fungus Gliocladium virens F051 and its Degradation Effect on Three Pathogens

作　　者：李姝江[1] 朱天辉[1] 徐春花[1] 韩珊[1] 李芳莲[1] 谯天敏[1] 杨伟[1] 黄琼[1] 

机构地区：[1]四川农业大学林学院,四川雅安625014

出　　处：《四川林业科技》2010年第3期65-70,共6页Journal of Sichuan Forestry Science and Technology

基　　金：国家自然科技资源共享平台(2005DKA21207-13)资助

摘　　要：系统研究了碳源、氮源、温度、起始pH值、培养基装量和接种量等因素对绿粘帚霉(Gliocladium virens)F051产几丁质酶的影响。结果表明,碳、氮源分别为麦芽糖和酵母粉最好,并通过正交试验法优化了发酵条件。优化后的产酶条件为:温度为28℃,pH值7,瓶装量为100 ml,每100 ml接种1 ml孢悬液,且碳源和氮源的量分别为1.5%和0.2%。从时间对菌丝生长和几丁质酶活的影响来看,培养第4天酶活便达到最高。在此基础上探索了几丁质对病原菌细胞壁的作用,结果显示,该几丁质酶粗酶液对3种病原真菌细胞壁有一定的降解效果,使病原真菌出现细胞壁变薄、断裂,质壁分离,原生质空泡等现象。In this paper,systematical studies were made of the effects of varied factors on chitinase-producing fungus Gliocladium virens F051,including carbon source,nitrogen source,cultural temperature,pH,bottled volume and inoculated volume.The results showed that the optimal carbon source and nitrogen source were maltose and yeast powder.By synthesizing the result of orthogonal design,the best fermentation conditions were got as follows： cultural temperature：28℃,pH 7,100 ml medium volume in 300ml triangle flask bulk,1ml inoculating volume,1.5% maltose and 0.2% yeast powder.According to the effect of time on the hyphae growth and chitinase,the chitinase activity would be the highest on the fourth day.On this basis,the effect of chitinase on three pathogens showed that it could degrade the cell-wall of the pathogens.The chitinase could make the pathogen cell-wall become thin or rupture,cell-wall and bioplasm separate,bioplasm become vacuolate and so on.

关 键 词：绿粘帚霉 几丁质酶 发酵条件 正交试验 

分 类 号：S476[农业科学—农业昆虫与害虫防治]