转化生长因子-β_1在中浓度氧致新生鼠支气管肺发育不良模型中的作用  

Effect of Transforming Growth Factor-β_1 in a Neonatal Mouse Bronchopulmonary Dysplasia Model Induced by Moderate Hyperoxia Exposure

作　　者：张志梅[1] 封志纯[2] 

机构地区：[1]山西医科大学,山西太原030001 [2]北京军区总医院附属八一儿童医院,北京100070

出　　处：《山西职工医学院学报》2010年第2期1-4,共4页Journal of Shanxi Medical College for Continuing Education

基　　金：国家自然科学基金项目(30772036)

摘　　要：目的:探讨中浓度氧(60%O2)暴露对新生小鼠发育的影响及损伤,并观察转化生长因子-β1(TGF-β1)在新生鼠慢性肺部疾病支气管肺发育不良(BPD)肺损伤中的作用。方法:30只新生雌性KM小鼠,随机分为实验组和对照组,每组15只。对照组置于空气中(FiO20.21);实验组置于封闭氧箱中(FiO20.6),制备中浓度氧致新生雌性小鼠BPD模型,记录吸氧后2 d、7 d、14 d、21 d、28 d小鼠体重,应用HE染色观察不同时间点HE染色肺组织病理改变,免疫组化技术观察TGF-β1蛋白表达水平。结果:实验组小鼠体重吸氧7 d后明显降低,HE染色下正常肺泡结构消失、肺泡融合、肺泡间隔增厚。高氧暴露72 h后,TGF-β1的表达升高,随着吸氧时间的增长TGF-β1的表达逐渐升高。结论:持续较高浓度吸氧可致新生雌性小鼠生长障碍和肺泡化阻滞,出现类似人类支气管肺发育不良的肺部改变。TGF-β1在肺纤维化的发生和持续过程中均发挥重要作用。Objective：To study the effect and injuries of moderate hyperoxia exposure（60% O2） on a neonatal mouse development to observe the effect of TGF-β1 on the lung injuries of the neonatal mouse bronchopulmonary dysplasia.Methods： Thirty neonatal female KM mice were randomly divided into an experiment group and a control group（n=15）.The control group was put in the air（FiO2 0.21） and the experiment group was put in the sealed oxygen box（FiO2 0.6） to prepare the model of the neonatal female mouse bronchopulmonary dysplasia induced by moderate hyperoxia,record the mouse body weight at the 2nd,7th,14th,21st,28th days after inhaling oxygen and observe the changes of pulmonary histopathology of different time points by HE staining and the expression levels of TGF-β1 protein by immunohistochemistry.Results： The body weight of the mouse in the experiment group was significantly reduced after 7 days of inhaling oxygen and the structure of normal alveolar disappeared,alveolar was fused and the alveolar intervals were thickened under the HE staining.The expression of TGF-β1 increased after 72 hours of hyperoxia exposure and the expression of TGF-β1 would continuously increase with the prolongation of inhaling oxygen.Conclusion：Oxygen inhalation of sustainable higher concentration may result in growth failure and pulmonary alveolarization block and appear pulmonary changes that is similarities to human bronchopulmonary dysplasia.TGF-β1 plays an important roles in development and sustainment of the pulmonary fibrosis（PF）.

关 键 词：高氧 支气管肺发育不良 肺损伤 转化生长因子-Β1 

分 类 号：R363.272[医药卫生—病理学]