病原菌细胞壁诱导下球毛壳菌葡聚糖酶活测定及抑菌试验  

Activity and Fungal Growth Inhibition Assay of β-1,3-Glucanase from Chaetomium globosum Induced by Pathogen Cell Walls

作　　者：陈刚[1] 杨谦[1] 刘志华[1] 彭鹏[1] 

机构地区：[1]哈尔滨工业大学生命科学与工程系,黑龙江哈尔滨150001

出　　处：《西南林学院学报》2010年第3期50-53,58,共5页Journal of Southwest Forestry College

基　　金：黑龙江省自然科学基金重点项目(ZJN03-04)资助

摘　　要：通过对峙培养试验,研究球毛壳菌对稻瘟病菌、尖孢镰刀菌、立枯丝核菌及核盘菌4种植物病原菌生长的抑制作用,结果表明,球毛壳菌对稻瘟病菌、尖孢镰刀菌、立枯丝核菌生长抑制作用更明显。在6种植物病原真菌细胞壁诱导下,球毛壳菌可产生β-1,3-葡聚糖酶,但是产酶规律不同。在稻瘟病菌、立枯丝核菌和尖孢镰刀菌细胞壁诱导下,β-1,3-葡聚糖酶活要明显高于杨树烂皮病菌、核盘菌和叶枯菌。其中,在稻瘟病菌细胞壁诱导11 d后,酶活达到最高,为0.61 U;而杨树烂皮病菌和叶枯菌细胞壁的诱导产酶效果最差。球毛壳菌可作为生物防治真菌。The inhibition effect of Chaetomium globosum on four pathogens i.e.,Pyricularia grisea,Fusarium oxysporum,Rhizoctonia solani,and Sclerotinia sclerotiorum was studied by using the fungal growth inhibition assay.The results showed that Chaetomium globosum could inhibit the growth of Rhizoctonia solani,Fusarium oxysporum and P.grisea more effectively.Chaetomium globosum could produce β-1,3-glucanase under induction with six plant pathogen cell walls,but the enzyme activities were different.The β-1,3-glucanase activity was significantly higher when induced with Rhizoctonia solan,Fusarium oxysporum and Pyricularia grisea cell walls rather than induced with Valsa sordida,Sclerotinia sclerotiorum and Septoria tritici cell walls.The highest activity obtained was 0.61U after 11 days induction with Pyricularia grisea cell walls,while the activities were respectively much lower under induction with Valsa sordida and Septoria tritici cell walls.All the results showed that Chaetomium globosum could inhibit the growth of some plant pathogens and one of the reasons for this should be that the C.globosum could be induced to produce β-1,3-glucanase which could degrade pathogen cell walls.Therefore,C.globosum can contribute to biocontrol of some fungi pathogens.

关 键 词：球毛壳菌 生物防治 Β-1 3-葡聚糖酶 

分 类 号：S763.15[农业科学—森林保护学]