缺血性脑损伤大鼠MicroRNA 210的动态变化  

Dynamic changes of mir-210 after brain stroke in rats

作　　者：高法梁[1] 娄远蕾[2] 阮琼芳[2] 涂伟[1] 吕世刚[1] 潘长福[1] 吴雷[1] 汪泱[2] 邓志锋[1] 

机构地区：[1]南昌大学第二附属医院神经外科,江西南昌330006 [2]南昌大学第一附属医院泌尿外科研究所,江西南昌330006

出　　处：《实验与检验医学》2010年第3期230-232,共3页Experimental and Laboratory Medicine

基　　金：国家自然科学基金资助项目(30960396);国家科技支撑项目(2008BAI68B06)

摘　　要：目的观察缺血性脑损伤后大鼠缺血皮质区microRNA 210的表达变化规律,探讨mir-210对脑缺血后血管新生的可能影响。方法实验分为缺血后1d、3d、7d组和假手术组(n=5),缺血组采用线栓法制作大脑中动脉阻塞模型,于缺血后1、3、7d处死大鼠,取缺血皮质区用于实验;假手术组仅暴露动脉。提取各实验组脑缺血皮质区的miRNA,Real time PCR比较各实验组mir-210的表达水平。结果脑缺血后mir-210表达显著上调,缺血后1d、3d、7d分别为假手术组的7.2±0.56、20.1±0.87和20.3±0.76倍(P<0.05)。结论脑缺血后mir-210表达显著上调,mir-210可能在促进脑缺血后血管新生过程中起重要作用。Objective To investigate the potential roles of mir-210 in angiogenesis after brain stroke by de-tecting its expression changes in impaired cortex after cerebral ischemia in rats.Methods The experiment rats was divided into four groups： 1,3,7 days post-ischemia groups and sham group.Middle cerebral artery occlusion（MCAO） rat models were made by obstructing MCA using a single strand nylon thread.Rats were killed at 1,3,7 days after ischemia,and the impaired cortex were extracted for examination.The sham group was not operated as control.MiRNAs were isolated from ischemic cortex and real time PCR was used to determine the expression of mir-210.Results Mir-210 expression was significantly increased in ischemic cortex after ischemia compared with sham group,with 7.2±0.56（1d）,20.1±0.87（3d） and 20.3±0.76（7d） fold changes respectively.Conclusions The expression of mir-210 shows significantly increased in ischemic cortex after ischemia,indicating a potential role in enhancing angiogenesis after brain ischemia.

关 键 词：脑缺血 微小RNA microRNA210 

分 类 号：R-332[医药卫生] Q522[生物学—生物化学]