气态甲醛对小鼠外周血白细胞的DNA损伤作用  

The injury of DNA in peripheral blood leukocytes of mice exposed to gaseous formaldehyde

作　　者：李兰[1] 程文文[1] 高娜娜[1] 常青[1] 熊国梅[1] 丁书茂[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2010年第2期284-287,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家科技支持计划项目(2006BAJ02A10)

摘　　要：为研究气态甲醛对外周血白细胞DNA的损伤作用,选用SPF级昆明种纯系雄性小鼠作为研究对象,用浓度为0.5 mg/m2、1.0 mg/m2、3.0 mg/m3气态甲醛对小鼠进行连续动态染毒72 h,染毒结束后,观察外周血白细胞的DNA断裂和DNA-蛋白质交联的状况.实验表明:气态甲醛能诱导小鼠外周血白细胞的DNA断裂,在各染毒浓度组与对照组均有及显著差异,在0.5 mg/m3浓度组DNA断裂效应比1.0 mg/m2,3.0 mg/m3浓度组高;1.0 mg/m2,3.0 mg/m3浓度组出现明显的DNA-蛋白质交联作用.研究结果表明:气态甲醛暴露对小鼠外周血白细胞的DNA有明显的损伤作用.For studying on DNA damage of peripheral blood leukocytes by gaseous formaldehyde, SPF-class Kun-Ming male mice were used to be exposed to gaseous formaldehyde in different concentrations： 0. 5 mg/m^-3,1.0 mg/m^-3 and 3.0 mg/m^-3. After 72 hours continuous dynamic inhalation, comet assay and adding protease K methods were used to observe DNA breakage and DNA-protein cross-linking situation of peripheral blood leukocytes. The results showed that gaseous formaldehyde induced DNA breakage in peripheral blood leukocytes, particularly at low concentrations of the apparent DNA- protein cross-linking role. And it proved that gaseous formaldehyde has significant damage effect on DNA in peripheral leukocytes of mice.

关 键 词：甲醛 彗星试验 DNA断裂 DNA蛋白质交联 外周血白细胞 

分 类 号：X171.5[环境科学与工程—环境科学] X592