COX-2及VEGF蛋白在大鼠乳腺癌骨移植瘤中的表达及意义  

Expressions of COX-2 and VEGF on a rat model of bone cancer initiated by MRMT-1 mammary gland carcinoma cells and its significance

作　　者：王日雄[1] 施烯[1] 林学德[1] 林建华[1] 

机构地区：[1]福建医科大学附属第一医院化疗科,骨肿瘤科,福建福州350004

出　　处：《中国癌症杂志》2010年第5期348-352,共5页China Oncology

基　　金：福建省卫生厅青年科研基金资助项目(项目编号:2006-2-11)

摘　　要：背景与目的:随着对生物学预后因子研究的不断深入,乳腺癌预后指标的联合检测和应用,能更有效、准确地估计患者的预后,并有助于指导乳腺癌的个体化治疗。本研旨在探讨究环氧化酶-2(cyclooxygenase-2,COX-2)及血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白对大鼠乳腺癌骨转移发生、发展的意义。方法:建立大鼠乳腺癌骨移植模型,观察正常组织及建模后肿瘤组织影像学、组织学变化,并检测COX-2、VEGF蛋白表达。结果:随着建模时间的延长,胫骨X线摄片及组织学显示肿瘤细胞向外侵蚀破坏骨皮质逐渐加强,COX-2、VEGF蛋白在肿瘤组织中表达的程度也逐渐增强,在正常组织及建模后第7、14和21天光密度值反映的表达量分别为916±258、1694±497、4304±890和7111±1069;930±268、1944±396、3226±819和4746±1032,两两比较差别均有显著的统计学意义(P<0.01)。结论:乳腺癌局部高表达COX-2蛋白可促进肿瘤细胞增殖,并通过上调VEGF蛋白水平,促进大鼠乳腺骨转移癌的发生和发展。Background and purpose：With further research of some prognostic biofactors,especially the study of combined detection and application of several biofactors and its combination with the clinic,the prognosis estimation in breast cancer has been made more efficient and accurate.This information is also helpful to individualized treatments in breast cancer.This study examines the expressions of the vascular endothelial growth factor（VEGF）and cyclooxygenase-2（COX-2）on a rat model of metastatic bone mammary gland cancer and evaluates the significance of this tumor invasion and metastasis.Methods：The rat model of metastatic bone cancer was initiated by intra鈥？tibial inoculations of the MRMT-1 rat鈥檚 mammary gland carcinoma cells in Sprague-Dawley rats.After establishing the model,segments of metastatic bone cancer were extracted to detect COX-2 and VEGF protein expression through the immunohistochemical SP method and the bone structural damage,respectively,and were monitored by radiological analysis and histology.Results：Seven,fourteen and twenty one days after establishing the model,X-ray and histology showed that tumor destroyed the bone more severely.The expressions of COX-2 and VEGF protein were 916卤258, 1 694卤497,4 304卤890,7 111卤1 069,930卤268,1 944卤396,3 226卤819 and 4 746卤1 032（P0.05）respectively in the normal tissue 7,14 and 21 days after establishing the model.Conclusion：A high expression of COX-2 in metastatic bone mammary gland cancer stimulates the proliferation of mammary gland cancer cells.COX-2 advances the level of VEGF to make the tumor angiogenesis and progression.

关 键 词：乳腺肿瘤 骨转移 环氧化酶-2 血管内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]