rAV-Tumstatin病毒载体构建及其生物活性检测  

Construction of rAV-Tumstatin viral vector and detection of its bioactivty

作　　者：任明[1] 刘禄成[1] 魏巍[1] 李志[1] 张明[1] 温都苏[1] 

机构地区：[1]吉林大学第二医院泌尿外科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2010年第3期453-455,607,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30972981)

摘　　要：目的:探讨腺病毒携带肿瘤抑素(rAV-Tumstatin)对人膀胱癌T24细胞株的影响与作用,为进一步研究其作用机制提供实验依据。方法:构建rAV-Tumstatin并感染人膀胱癌T24细胞株,将细胞分为rAV-Tumstatin组、rAV-MCS空病毒组和空白对照组,采用噻唑蓝(MTT)比色法和原位末端标记(TUNEL)法分别检测感染细胞的增殖和凋亡情况。结果:rAV-Tumstatin既可以稳定感染T24细胞也可以在该细胞内表达携带的外源基因EGFP。TUNEL法检测,rAV-Tumstatin组细胞凋亡率为32.9%,rAV-MCS空病毒组和空白对照组分别为13.5%和6.7%,3组间比较差异均有显著性(P<0.01)。MTT比色法检测T24细胞株的增殖生长能力,rAV-Tumstatin组G0/G1期60.3%,S期17.4%,G2/M期21.5%;空病毒组G0/G1期45.2%,S期29.5%,G2/M期24.8%;对照组G0/G1期43.7%,S期32.8%,G2/M期23.7%。3组间各细胞周期细胞增殖率比较差异有显著性(P<0.05)。结论:rAV-Tumstatin能抑制膀胱癌T24细胞株增殖并诱导其凋亡,对膀胱癌基因治疗的研究具有一定的价值。Objective To investigate the influence and effect of rAV-Tumstatin on T24 cell lines,and provide experimental basis for further study on its mechanism.Methods rAV-Tumstatin was constructed and the T24 cell lines were infected.The cells were divided into rAV-Tumstatin group,vacant viral infection group and control group.The proliferation and apoptosis of the infected cells were detected by MTT and TUNEL.Results The T24 cells could be stably infected by rAV-Tumstatin and expressed the exogenous gene EGFP. The result of TUNEL showed the apoptotic rates in rAV-Tumstatin group,vacant viral infection group and control group were 32.9%,13.5%,and 6.7%,respectively;there were significant differences between three groups（P0.01）.The result of MTT which detected the proliferation and growth of T24 cells respectively showed as follows：rAV-Tumstatin group,G0/G1 60.3%,S 17.4%,G2/M 21.5%;vacant viral infection group,G0/G1 45.2%,S 29.5%,G2/M 24.8%; control group,G0/G1 43.7%,S 32.8%,G2/M 23.7%;there were also significant differences between three groups（P0.05）.Conclusion rAV-Tumstatin can inhibit the proliferation and induce the apoptosis of T24 cells,and it is very valuable for the gene therapy of bladder cancer.

关 键 词：膀胱肿瘤 重组腺病毒 肿瘤抑素 

分 类 号：R735.14[医药卫生—肿瘤]