血清人附睾蛋白4的检测及其在卵巢癌诊断中的意义  

Detection of serum human epididymis protein 4 and its significance in diagnosis of ovarian cancer

作　　者：欧健[1] 张晓霞[1] 王翠翠[1] 王丽娜[1] 

机构地区：[1]吉林大学第一医院妇产科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第3期543-545,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200705155);吉林省教育厅基金资助课题(吉教科合字[2005]第132号)

摘　　要：目的:检测卵巢癌患者血清人附睾蛋白4(HE4)水平,探讨血清HE4在卵巢癌诊断中的意义。方法:应用ELISA法检测卵巢癌组、卵巢良性肿瘤组、妇科其他恶性肿瘤组、其他器官恶性肿瘤组患者血清HE4水平;观察卵巢癌组手术前、后血清HE4水平变化;比较卵巢癌组血清HE4和CA125水平在卵巢癌诊断中的作用。结果:卵巢癌组血清HE4水平和阳性率分别为(242.76±113.40)pmol·L-1和70.59%,明显高于卵巢良性肿瘤组[(85.57±63.40)pmol·L-1和7.02%]、妇科其他恶性肿瘤组[(73.16±12.27)pmol·L-1和0.00%]、其他器官恶性肿瘤组[(80.65±16.24)pmol.L-1和0.00%]及对照组[(43.95±18.16)pmol·L-1和0.00%](P<0.01);卵巢癌组术后血清HE4水平为(134.83±56.20)pmol·L-1,显著低于手术前HE4水平(242.76±113.40)pmol·L-1(P<0.05);早期卵巢癌血清HE4阳性率高于血清CA125阳性率;卵巢癌组血清HE4和CA125联合检测的灵敏性高于二者单独检测,特异性低于二者的单独检测(P<0.05)。结论:HE4在卵巢癌诊断中有重要意义,尤其在早期卵巢癌的诊断中其灵敏度及特异性均优于CA125,二者联合检测可提高卵巢癌的早期诊断率。Objective To detect the level of serum human epididymis protein 4（HE4） in the patients with ovarian cancer and approach the significance of serum HE4 in the diagnosis of ovarian cancer.Methods ELISA mothod was used to detect the levels of serum HE4 in ovarian cancer group,benign ovarian tumor group,other malignant gynecology tumor group and malignant tumor of other organs group.The changes of the serum HE4 level in ovarian cancer group before and after operation were detected.The effects of the serum HE4 level and CA215 level in ovarian cancer group in the diagnois of ovarian cancer were compared.Results The serum HE4 levels ovarian cancer group was （242.76±113.40）pmol·L-1 and the positive rate was 70.59%,they were higher than those in benign ovarian tumor group（85.57 pmol·L-1±63.40 pmol·L-1,7.02%）,other malignant gynecology tumor group （73.16 pmol·L-1±12.27 pmol·L-1,0.00%）,malignant tumor of other organs group（80.65 pmol·L-1±16.24 pmol·L-1,0.00%）and control group（43.95 pmol·L-1±18.16 pmol·L-1,0.00%）（P0.01）;the serum HE4 level in ovarian cancer group after operation（134.83 pmol·L-1±56.20 pmol·L-1） was lower than before operation（242.76 pmol·L-1±113.40 pmol·L-1）（P0.05）.The positive rate of serum HE4 in the early ovarian cancer was higher than that of seum CA125.The sensitiveity of HE4 combined with CA125 was higher than each of them,the specificity of HE4 combined with CA125 was lower than each of them（P0.05）.Conclusion The HE4 serum has important significance in the diagnosis of ovarian cancer,especially,its sensitivity and specificity are all better than CA125 in the diagnosis of early ovarian cancer,the detection of HE4 combined with CA125 can elevate the early diagnosis rate of ovarian cancer.

关 键 词：人附睾蛋白4 CA125 卵巢肿瘤 酶联免疫法 

分 类 号：R737.31[医药卫生—肿瘤]