原因不明复发性流产中CD4^+ Notch1^+ T细胞与IL-10的相关性分析  

Relationship Between CD4^+ Notch1^+ T Cells and IL-10 Expression in Unexplained Recurrent Spontaneous Abortion

作　　者：印永祥[1] 韩晓枫[2] 赵益明 何杨 

机构地区：[1]南京医科大学附属无锡市妇幼保健院病理科,江苏无锡214002 [2]南京医科大学基础医学院,江苏南京210029 [3]江苏省血液研究所,江苏苏州215006

出　　处：《标记免疫分析与临床》2010年第3期157-160,共4页Labeled Immunoassays and Clinical Medicine

基　　金：2008年度无锡市卫生系统指令性科研项目(编号XM0802)资助

摘　　要：探讨原因不明复发性流产(URSA)中CD4+Notch1+T细胞(Notch1+占CD4+T细胞的比例)与白介素-10(IL-10)表达水平的相关性。以雌性CBA/J×雄性Balb/c为正常妊娠模型,以雌性CBA/J×雄性DBA/2J为自然流产模型,采用流式细胞术检测6例未孕CBA/J雌性小鼠、6例正常妊娠模型孕14天CBA/J雌性小鼠和6例自然流产模型孕14天CBA/J雌性小鼠脾细胞中CD4+Notch1+T细胞,同时运用ELISA法检测其血清中IL-10的表达水平。相比于未孕组,正常妊娠模型中CD4+Notch1+T细胞比例减少,而自然流产模型中CD4+Notch1+T细胞比例增加,正常妊娠模型与自然流产模型中CD4+Notch1+T细胞比例差异有统计学意义(P<0.05)。CD4+Notch1+T细胞比例与IL-10负相关(r=-0.568,P<0.05)。结论:CD4+Notch1+T细胞可能参与原因不明复发性流产发病机制,拮抗Notch1+表达有可能成为治疗URSA新途径。To investigate the relationship between CD4＋Notch1＋ T cells and IL-10 expression in unexplained recurrent spontaneous abortion（URAS）,the female CBA/J× male BALB/c matings were used as the normal pregnancy model and female CBA/J× male DBA/2J matings were used as the abortion-prone model.The serum IL-10 and the proportions of CD4＋ Notch1＋ T cells from spleen of six virgin CBA/J mice（control group）,six pregnant CBA/J mice mated with DBA/2J males（URSA group） and six pregnant CBA/J mice mated with Balb/c males at day 14 of gestation（normal pregnant group） were assessed by ELISA and flow cytometry respectively.The linear correlation analysis of IL-10 and CD4＋Notch1＋ T cells was conducted.The results showed that the proportions of CD4＋ Notch1＋T cells of normal pregnant group was decreased,but the proportions of CD4＋ Notch1＋T cells of RSA group was increased compared with control group.Compared with normal pregnant group,the proportions of CD4＋Notch1＋ T cells of RSA group was decreased significantly（P0.05）.The proportion of CD4＋Notch1＋ T cells was negatively correlated with that of IL-10（r=-0.568,P0.05）.The results indicate that CD4＋Notch1＋T cells may participate in pathogenesis of unexplained recurrent spontaneous abortion,and rivalry of Notch1＋ expression may be a new pathway for URSA therapy.

关 键 词：小鼠妊娠模型 复发性流产 白介素10 NOTCH1 

分 类 号：R714.21[医药卫生—妇产科学]