甘松对青霉素致痫大鼠行为学表现及脑电图的影响  

The efficacy of nardostachyos in the theatment of epileptic rat induced by penicillin

作　　者：丁莉[1] 李新玲[1] 邵良[1] 朱连海[1] 

机构地区：[1]南通大学第二附属医院神经内科,江苏南通226001

出　　处：《癫痫与神经电生理学杂志》2010年第3期132-135,共4页Journal of Epileptology and Electroneurophysiology(China)

基　　金：江苏省南通市科技计划资助项目（S2006020）

摘　　要：目的：观察甘松对青霉素致痫大鼠的疗效。方法：将50只Wistar大鼠随机分为5组，每组10只，分别为正常对照组（Ⅰ组）和4个青霉素致痼模型组，即单纯模型组（Ⅱ组）、用甘松预处理组（Ⅲ组）、用丙戊酸钠预处理组（Ⅳ组）和用甘松合并丙戊酸钠预处理组（Ⅴ组）。甘松预处理组灌服甘松62．5mg／（kg·d），丙戊酸钠预处理组灌服丙戊酸钠0．64mg／（kg·d），甘松合并丙戊酸钠预处理组灌胃剂量为甘松62．5mg／（kg·d）加丙戊酸钠o．32nag／（kg·d），正常对照组和单纯模型组灌服等体积的生理盐水。每组大鼠每天灌胃一次，连续14d。灌胃第14天除正常对照组外其余4组大鼠腹腔注射青霉素500万U／kg诱导大鼠急性癫痫发作，观察和比较各组大鼠行为及脑电图变化。结果：与单纯模型组比较，甘松预处理组、丙戊酸钠预处理组、甘松合并丙戊酸钠预处理组可延长癫痫大鼠惊厥发作潜伏期，明显减轻大鼠痫性发作程度，改善大鼠大脑皮层脑电图，其中以甘松合并丙戊酸钠预处理组最为明显。结论：甘松对青霉素致痫大鼠具有一定的抗癫痫作用，与丙戊酸钠联用有协同作用。Objective：To study the efficacy of Nardostachyos used for the pre-treatment in generalized epileptic rat models induced by penicillin. Methods： Fifty Wistar rats were randomly divided into five groups （10 each） ：the normal control group（Group Ⅰ） ,and 4 epileptic rat model groups induced by peniciUin：the mere epileptic model group（Group Ⅱ）, the Nardostachyos pre-treatment group（Group Ⅲ） ,the sodium valproate pre-treatment group（GroupⅣ）, the Nardostachyos and sodium valproate pre-treatment group（Group Ⅴ ）. The rats in GroupⅢ were drenched with Nardostachyos at a dose of 62.5 mg/（kg·d） ,the rats in group IV were drenched with sodium valproate at a dose of 0.64 g/（kg·d） and the rats in Group V were drenched with nardostachyos62.5 mg/（kg·d） and sodinm valproate0.32 g/（kg·d） and （Group Ⅰ）and （Group Ⅱ） were pre-treated with isovolumic normal saline. Each above mentianed pretreatment last 14 days. Then on the fourteenth day the rats in all above groups were injected intraperitoneally with penicillin （5000000 U/kg）. The changes in epileptic behavior and electroencephalograms were observed and compared within the five groups. Results： Compared with mere epileptic model group, the Nardostaehyos pre-treatment group,the sodium valproate pre-treatment group and the group pre-treated with Nardostachyos and sodium valproate showed a prolonged latency of epileptic attack and reduced grades of epileptic behavior. And the rates of severe and mediate abnormality of EEG reduced,in which the group pre-treated with Nardostachyos and sodium valproate were most obvious. Conclusion ： The administration of Nardostachyos has a definite efficacy for the pre-treatment of generalized epileptic rats models induced by penicillin. The combined administration of Nardostachyos with sodium valproate has synergistic function.

关 键 词：甘松:丙戊酸钠 大鼠 癫痫 青霉素 癫痫模型 

分 类 号：R742.1[医药卫生—神经病学与精神病学] Q95-33[医药卫生—临床医学]