卵圆细胞在实验性肝癌发生中的作用  

Study on the Role of Oval Cells in Experimental Hepatocellular Carcinogenesis

作　　者：薛玲[1] 赵国强[1] 吴惠茜[1] 董郡[1] 

机构地区：[1]中山医科大学病理学教研室

出　　处：《中山医科大学学报》1999年第1期36-38,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：卫生部回国人员启动基金;国家自然科学基金

摘　　要：目的：观察诱癌过程中ｃＨａｒａｓ、ｐ２１蛋白、ＡＦＰ及ＰＣＮＡ在肝细胞及肝卵圆细胞中的表达变化。方法：用化学致癌剂３′甲基４二甲基氨偶氮苯（３′ｍｅｔｈｙ１４ｄｉｍｅｔｈｙｌａｍｉｎｏａｚｏｂｅｎｚｅｎｅ，３′ＭｅＤＡＢ）诱发ＳＤ大鼠肝癌，通过免疫组化、原位杂交等方法检测各相关因子在肝细胞及肝卵圆细胞内的表达。结果：诱癌４周，大鼠肝内出现大量的卵圆细胞，呈ＰＣＮＡ强阳性。６～８周，部分增生的卵圆细胞ｐ２１ｒａｓ及ＡＦＰ阳性反应。原位杂交显示，诱癌的第４～８周，ｃＨａｒａｓ的阳性表达细胞呈逐渐增多趋势；１４周时，ｃＨａｒａｓｍＲＮＡ阳性表达细胞减少；至１７周，所有癌组织内显示ｃＨａｒａｓ基因表达阴性，而癌旁组织内则可见ｒａｓ基因的大量表达。结论：卵圆细胞是宿主受化学致癌剂作用后发生肝癌的始动细胞，与肝癌的发生有着密切的联系。Objective: To observe the expression of c Ha ras,p21,AFP and PCNA in oval cells and liver cells in the process of hepatocarcinogenesis. Methods:Male SD rats are used as experimental animals and the animal model of experimental hepatocarcinoma is established by means of 3′ me DAB administration.The expression of c Ha ras,p21,AFP and PCNA are detected by immunohistochemistry and in situ hybridization. Results:Large amount of PCNA positive oval cells appeared in the 4th week of cancer induction.In 6th and 8th week,positive reaction of p21 ras and AFP appeared in some hyperplastic oval cells.The results of in situ hybridization showed,from 4th to 8th week the number of c Ha ras positive cells increased gradually.In 14th week the number of c Ha ras positive cells decreased.In 17th week the positive signal of ras gene expression could be seen only in paraneoplastic tissue, and in neoplastic tissue no positive signal could be detected. Conclusion:The oval cells might be the initiated cells of liver carcinoma of host rats fed by chemical carcinogen and have a close relationship with histogenesis of liver carcinoma.

关 键 词：肝肿瘤 癌基因 卵圆细胞 实验研究 

分 类 号：R735.702[医药卫生—肿瘤]