GC-MS分析法在人参有机氯农药残留检测中的应用  

Application of gas chromatography mass spectrometry for determination of the organochlorine pesticide residues in Ginseng

作　　者：赵云峰[1,2] 陈建民[1,2] 王绪卿[1,2] 

机构地区：[1]中国预防医学科学院营养与食品卫生研究所 [2]中国医学科学院药用植物研究所

出　　处：《卫生研究》1999年第1期53-53,共1页Journal of Hygiene Research

摘　　要：以丙酮－饱和氯化钠溶液－石油醚的一步法提取分离技术制备样品溶液，采用ＧＣ－ＭＳ分析方法对人参中有机氯农药残留进行了测定。加标人参样品的测定结果表明：两个水平加标回收率：０．１０ｍｇ／ｋｇ添加水平时，除ＰＣＮＢ平均回收率为５８．３％，其它组分的平均回收率为７６．０％～９０．１％，相对标准差为９．３％～１５．０％；０．０５０ｍｇ／ｋｇ添加水平时，平均回收率为６２．３％～８７．２％，相对标准差为６．０％～２２．５％。应用本方法测定人参中有机氯农药残留，基本上满足了灵敏、快速。A gas chromatographic electronionization mass spectrometric method (GC EIMS) was developed for the determination of organochlorine pesticide residues in Ginseng plants. The Ginseng samples were extracted with acetone, paritioned using petroleam ester with sodium chloride saturated aqueous solution and clean up with concentrated sulfuric acid. The organochlorine pesticide residues were analysed by GC MS with monitoring selective mass ions. Ten organochlorine pesticides including alpha, beta, gamma and delta isomers of hexachlorocyclohexane (HCH), pp' DDE, pp' DDT, op' DDT, pp' DDT, pentachloronitrobenzene (Quintozene, PCNB), and hexachlorobenzene(HCB), were determined simultaneously. Targeted pesticide analytes were separated on DB 5 capillary column. The optimum chromatographic conditions and MS parameters were determined. The average recovery rates for those pesticide residues Ginseng were from 76 6% to 90 1% at 0 1mg/kg of spiked level, except 58 3% for PCNB. The relative standard deviations were 9 3%～15 0%.

关 键 词：有机氯农药 人参 农药残留 GC-MS分析法 

分 类 号：R282.71[医药卫生—中药学] TS207.53[医药卫生—中医学]